ArticleNucleic acids research2011
Cross-linking of DNA through HMGA1 suggests a DNA scaffold.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
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- Multidimensional scaling methods can reconstruct genomic DNA loops using Hi-C data properties.PloS one · 2023Article
- Hmga2 protein loss alters nuclear envelope and 3D chromatin structure.BMC biology · 2022Article
- Chromatin Architectural Factors as Safeguards against Excessive Supercoiling during DNA Replication.International journal of molecular sciences · 2020Review
- p53 CRISPR Deletion Affects DNA Structure and Nuclear Architecture.Journal of clinical medicine · 2020Article
- Adenovirus-Mediated Delivery of Decoy Hyper Binding Sites Targeting Oncogenic HMGA1 Reduces Pancreatic and Liver Cancer Cell Viability.Molecular therapy oncolytics · 2018Article
- Article
- Binding of high mobility group A proteins to the mammalian genome occurs as a function of AT-content.PLoS genetics · 2017Article
- Oncofetal HMGA2 effectively curbs unconstrained (+) and (-) DNA supercoiling.Scientific reports · 2017Article
- The Global Relationship between Chromatin Physical Topology, Fractal Structure, and Gene Expression.Scientific reports · 2017Article
- Cell-based high-throughput compound screening reveals functional interaction between oncofetal HMGA2 and topoisomerase I.Nucleic acids research · 2016Article
- Proliferation-dependent positioning of individual centromeres in the interphase nucleus of human lymphoblastoid cell lines.Molecular biology of the cell · 2015Article
- Post-transcriptional regulatory elements and spatiotemporal specification of neocortical stem cells and projection neurons.Neuroscience · 2013Review
- P2TF: a comprehensive resource for analysis of prokaryotic transcription factors.BMC genomics · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Binding of proteins to DNA is usually considered 1D with one protein bound to one DNA molecule. In principle, proteins with multiple DNA binding domains could also bind to and thereby cross-link different DNA molecules. We have investigated this possibility using high-mobility group A1 (HMGA1) proteins, which are architectural elements of chromatin and are involved in the regulation of multiple DNA-dependent processes. Using direct stochastic optical reconstruction microscopy (dSTORM), we could show that overexpression of HMGA1a-eGFP in Cos-7 cells leads to chromatin aggregation. To investigate if HMGA1a is directly responsible for this chromatin compaction we developed a DNA cross-linking assay. We were able to show for the first time that HMGA1a can cross-link DNA directly. Detailed analysis using point mutated proteins revealed a novel DNA cross-linking domain. Electron microscopy indicates that HMGA1 proteins are able to create DNA loops and supercoils in linearized DNA confirming the cross-linking ability of HMGA1a. This capacity has profound implications for the spatial organization of DNA in the cell nucleus and suggests cross-linking activities for additional nuclear proteins.
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