ArticleBMC genomics2011
BAC library resources for map-based cloning and physical map construction in barley (Hordeum vulgare L.).
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Genotyping by Sequencing Advancements in Barley.Frontiers in plant science · 2022Review
- History and future perspectives of barley genomics.DNA research : an international journal for rapid publication of reports on genes and genomes · 2020Review
- A Tale of Two Families: Whole Genome and Segmental Duplications Underlie Glutamine Synthetase and Phosphoenolpyruvate Carboxylase Diversity in Narrow-Leafed Lupin (International journal of molecular sciences · 2020Article
- Leaf Variegation and Impaired Chloroplast Development Caused by a Truncated CCT Domain Gene inThe Plant cell · 2019Article
- Mutations in the gene of the Gα subunit of the heterotrimeric G protein are the cause for theHereditas · 2018Article
- Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum.PloS one · 2018Article
- Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.Scientific data · 2017Article
- Multiplex sequencing of bacterial artificial chromosomes for assembling complex plant genomes.Plant biotechnology journal · 2016Article
- A Homolog of Blade-On-Petiole 1 and 2 (BOP1/2) Controls Internode Length and Homeotic Changes of the Barley Inflorescence.Plant physiology · 2016Article
- The Cer-cqu gene cluster determines three key players in a β-diketone synthase polyketide pathway synthesizing aliphatics in epicuticular waxes.Journal of experimental botany · 2016Article
- Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- The barley Uniculme4 gene encodes a BLADE-ON-PETIOLE-like protein that controls tillering and leaf patterning.Plant physiology · 2015Article
- High-resolution genetic mapping and physical map construction for the fertility restorer Rfm1 locus in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- Characterization of rubber tree microRNA in phytohormone response using large genomic DNA libraries, promoter sequence and gene expression analysis.Molecular genetics and genomics : MGG · 2014Article
- The barley Frost resistance-H2 locus.Functional & integrative genomics · 2014Article
- PROTEIN DISULFIDE ISOMERASE LIKE 5-1 is a susceptibility factor to plant viruses.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Construction and characterization of a bacterial artificial chromosome library for the hexaploid wheat line 92R137.BioMed research international · 2014Article
- A sequence-ready physical map of barley anchored genetically by two million single-nucleotide polymorphisms.Plant physiology · 2014Article
- Conserved synteny-based anchoring of the barley genome physical map.Functional & integrative genomics · 2013Article
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11 authors.
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Abstract
backgroundAlthough second generation sequencing (2GS) technologies allow re-sequencing of previously gold-standard-sequenced genomes, whole genome shotgun sequencing and de novo assembly of large and complex eukaryotic genomes is still difficult. Availability of a genome-wide physical map is therefore still a prerequisite for whole genome sequencing for genomes like barley. To start such an endeavor, large insert genomic libraries, i.e. Bacterial Artificial Chromosome (BAC) libraries, which are unbiased and representing deep haploid genome coverage, need to be ready in place.
resultFive new BAC libraries were constructed for barley (Hordeum vulgare L.) cultivar Morex. These libraries were constructed in different cloning sites (HindIII, EcoRI, MboI and BstXI) of the respective vectors. In order to enhance unbiased genome representation and to minimize the number of gaps between BAC contigs, which are often due to uneven distribution of restriction sites, a mechanically sheared library was also generated. The new BAC libraries were fully characterized in depth by scrutinizing the major quality parameters such as average insert size, degree of contamination (plate wide, neighboring, and chloroplast), empty wells and off-scale clones (clones with <30 or >250 fragments). Additionally a set of gene-based probes were hybridized to high density BAC filters and showed that genome coverage of each library is between 2.4 and 6.6 X.
conclusionBAC libraries representing >20 haploid genomes are available as a new resource to the barley research community. Systematic utilization of these libraries in high-throughput BAC fingerprinting should allow developing a genome-wide physical map for the barley genome, which will be instrumental for map-based gene isolation and genome sequencing.
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