ArticleProceedings of the National Academy of Sciences of the United States of America2011
Forming giant vesicles with controlled membrane composition, asymmetry, and contents.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
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Who cites it
67 citing papers in PubMed.
- Reversible Charge-Mediated Capture and Release of Extracellular Vesicles Using Giant Unilamellar Vesicles.ACS omega · 2026Article
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Cannabidiol (CBD) Induces Lipid Microdomain Disruption or Budding in Ternary Mixtures.The journal of physical chemistry. B · 2026Article
- Mechanical performance of hybrid polymer-lipid vesicles with leaflet asymmetry engineered using microfluidics.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Genetically programmed synthetic cells for thermo-responsive protein synthesis and cargo release.Nature chemical biology · 2024Article
- Effect of leaflet asymmetry on the stretching elasticity of lipid bilayers with phosphatidic acid.Biophysical journal · 2024Article
- Coupling liquid phases in 3D condensates and 2D membranes: Successes, challenges, and tools.Biophysical journal · 2024Review
- SecYEG-mediated translocation in a model synthetic cell.Synthetic biology (Oxford, England) · 2024Article
- Microfluidics-mediated Liposomal Nanoparticles for Cancer Therapy: Recent Developments on Advanced Devices and Technologies.Current topics in medicinal chemistry · 2024Review
- Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many.ACS synthetic biology · 2023Review
- Biomimetic asymmetric bacterial membranes incorporating lipopolysaccharides.Biophysical journal · 2023Article
- Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer.Nature methods · 2023Article
- A Guide to Your Desired Lipid-Asymmetric Vesicles.Membranes · 2023Review
- A Review of Optical Imaging Technologies for Microfluidics.Micromachines · 2022Review
- Review
- Membrane-associated phase separation: organization and function emerge from a two-dimensional milieu.Journal of molecular cell biology · 2021Review
- Optimized cDICE for Efficient Reconstitution of Biological Systems in Giant Unilamellar Vesicles.ACS synthetic biology · 2021Article
- Single Cell-like Systems Reveal Active Unidirectional and Light-Controlled Transport by Nanomachineries.ACS nano · 2021Article
- An integrated microfluidic platform to fabricate single-micrometer asymmetric giant unilamellar vesicles (GUVs) using dielectrophoretic separation of microemulsions.Biomicrofluidics · 2021Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Growing knowledge of the key molecular components involved in biological processes such as endocytosis, exocytosis, and motility has enabled direct testing of proposed mechanistic models by reconstitution. However, current techniques for building increasingly complex cellular structures and functions from purified components are limited in their ability to create conditions that emulate the physical and biochemical constraints of real cells. Here we present an integrated method for forming giant unilamellar vesicles with simultaneous control over (i) lipid composition and asymmetry, (ii) oriented membrane protein incorporation, and (iii) internal contents. As an application of this method, we constructed a synthetic system in which membrane proteins were delivered to the outside of giant vesicles, mimicking aspects of exocytosis. Using confocal fluorescence microscopy, we visualized small encapsulated vesicles docking and mixing membrane components with the giant vesicle membrane, resulting in exposure of previously encapsulated membrane proteins to the external environment. This method for creating giant vesicles can be used to test models of biological processes that depend on confined volume and complex membrane composition, and it may be useful in constructing functional systems for therapeutic and biomaterials applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.