ArticleNucleic acids research2011
Quadruplex formation is necessary for stable PNA invasion into duplex DNA of BCL2 promoter region.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- The G-quadruplex ligand CX-5461: an innovative candidate for disease treatment.Journal of translational medicine · 2025Review
- Targeting a KRAS i-motif forming sequence by unmodified and gamma-modified peptide nucleic acid oligomers.Biopolymers · 2023Article
- Impact of G-Quadruplexes on the Regulation of Genome Integrity, DNA Damage and Repair.Biomolecules · 2021Review
- Beyond small molecules: targeting G-quadruplex structures with oligonucleotides and their analogues.Nucleic acids research · 2021Review
- G-quadruplex-R-loop interactions and the mechanism of anticancer G-quadruplex binders.Nucleic acids research · 2020Review
- Selective targeting of mutually exclusive DNA G-quadruplexes: HIV-1 LTR as paradigmatic model.Nucleic acids research · 2020Article
- Selective recognition of c-MYC Pu22 G-quadruplex by a fluorescent probe.Nucleic acids research · 2019Article
- The effects of DNA supercoiling on G-quadruplex formation.Nucleic acids research · 2017Article
- Nucleic acid clamp-mediated recognition and stabilization of the physiologically relevant MYC promoter G-quadruplex.Nucleic acids research · 2016Article
- Chemical modifications of artificial restriction DNA cutter (ARCUT) to promote its in vivo and in vitro applications.Artificial DNA, PNA & XNA · 2014Review
- PPG peptide nucleic acids that promote DNA guanine quadruplexes.Chembiochem : a European journal of chemical biology · 2014Article
- Cytotoxic effects and specific gene expression alterations induced by I-125-labeled triplex-forming oligonucleotides.International journal of radiation biology · 2012Article
- Targeting DNA G-quadruplex structures with peptide nucleic acids.Current pharmaceutical design · 2012Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Guanine-rich sequences are highly abundant in the human genome, especially in regulatory regions. Because guanine-rich sequences have the unique ability to form G-quadruplexes, these structures may play a role in the regulation of gene transcription. In previous studies, we demonstrated that formation of G-quadruplexes could be induced with peptide nucleic acids (PNAs). PNAs designed to bind the C-rich strand upstream of the human BCL2 gene promoted quadruplex formation in the complementary G-rich strand. However, the question whether G-quadruplex formation was essential for PNA invasion remained unanswered. In this study, we compared PNA invasion in the native and mutant, i.e. not forming G-quadruplex, BCL2 sequences and showed that G-quadruplex is required for effective PNA invasion into duplex DNA. This finding provides strong evidence for not only sequence-specific, but also quadruplex specific, gene targeting with PNA probes. In addition, we examined DNA-duplex invasion potential of PNAs of various charges. Using the gel shift assay, chemical probing and dimethyl sulfate (DMS) protection studies, we determined that uncharged zwitterionic PNA has the highest binding specificity while preserving efficient duplex invasion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.