ArticleMicrobial cell factories2011
Improved triacylglycerol production in Acinetobacter baylyi ADP1 by metabolic engineering.
Article in Microbial cell factories, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 113 citations in OpenAlex.
- Mixed Waste Streams for Bioproduction: Exploring Bacterial Wax Ester Production in Nitrogen-Rich Acidogenic Fermentate.Microbial biotechnology · 2026Article
- Engineering microbial consortia for mixed plastic upcycling.Nature communications · 2025Article
- Assessment of lignocellulose hydrolysate component consumption by co-culture of Acinetobacter baylyi ADP1 and Lipomyces starkeyi Y-1389.Archives of microbiology · 2025Article
- Conversion and upgrading of syringate by Acinetobacter baylyi ADP1.Microbial cell factories · 2025Article
- Rewiring Aromatic Compound Consumption: Chromosomal Amplification and Evolution of a Foreign Pathway inACS synthetic biology · 2025Article
- Cis,cis-muconic acid production from lignin related molecules byAcinetobacter baylyi ADP1.Microbial cell factories · 2025Article
- Metabolic engineering ofMetabolic engineering communications · 2024Article
- Carbon-wise utilization of lignin-related compounds by synergistically employing anaerobic and aerobic bacteria.Biotechnology for biofuels and bioproducts · 2024Article
- Enhanced upgrading of lignocellulosic substrates by coculture of Saccharomyces cerevisiae and Acinetobacter baylyi ADP1.Biotechnology for biofuels and bioproducts · 2024Article
- Article
- Engineering cell morphology by CRISPR interference in Acinetobacter baylyi ADP1.Microbial biotechnology · 2022Article
- Characterization of Highly Ferulate-Tolerant Acinetobacter baylyi ADP1 Isolates by a Rapid Reverse Engineering Method.Applied and environmental microbiology · 2022Article
- Endophytes in Lignin Valorization: A Novel Approach.Frontiers in bioengineering and biotechnology · 2022Review
- EngineeringMetabolic engineering communications · 2021Article
- Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site.Microbiology (Reading, England) · 2021Article
- Characterization ofMicroorganisms · 2021Article
- Wax ester production in nitrogen-rich conditions by metabolically engineeredMetabolic engineering communications · 2020Article
- Development of a genetic toolset for the highly engineerable and metabolically versatile Acinetobacter baylyi ADP1.Nucleic acids research · 2020Article
- Rapid and assured genetic engineering methods applied to Acinetobacter baylyi ADP1 genome streamlining.Nucleic acids research · 2020Article
- Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules.Microbial cell factories · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTriacylglycerols are used in various purposes including food applications, cosmetics, oleochemicals and biofuels. Currently the main sources for triacylglycerol are vegetable oils, and microbial triacylglycerol has been suggested as an alternative for these. Due to the low production rates and yields of microbial processes, the role of metabolic engineering has become more significant. As a robust model organism for genetic and metabolic studies, and for the natural capability to produce triacylglycerol, Acinetobacter baylyi ADP1 serves as an excellent organism for modelling the effects of metabolic engineering for energy molecule biosynthesis.
resultsBeneficial gene deletions regarding triacylglycerol production were screened by computational means exploiting the metabolic model of ADP1. Four deletions, acr1, poxB, dgkA, and a triacylglycerol lipase were chosen to be studied experimentally both separately and concurrently by constructing a knock-out strain (MT) with three of the deletions. Improvements in triacylglycerol production were observed: the strain MT produced 5.6 fold more triacylglycerol (mg/g cell dry weight) compared to the wild type strain, and the proportion of triacylglycerol in total lipids was increased by 8-fold.
conclusionsIn silico predictions of beneficial gene deletions were verified experimentally. The chosen single and multiple gene deletions affected beneficially the natural triacylglycerol metabolism of A. baylyi ADP1. This study demonstrates the importance of single gene deletions in triacylglycerol metabolism, and proposes Acinetobacter sp. ADP1 as a model system for bioenergetic studies regarding metabolic engineering.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.