ArticleThe journal of physical chemistry. B2011
Bioelectronic delivery of electrons to cytochrome P450 enzymes.
Article in The journal of physical chemistry. B, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Linking Nanoscale Film Properties to Electrochemical Response in Cytochrome P450 Membrane Enzyme Films.ACS measurement science au · 2026Article
- High-throughput Optical Analysis to Inform Design of Electrochemical Biosensors.ACS measurement science au · 2026Review
- Electrochemical Probing of Human Liver Subcellular S9 Fractions for Drug Metabolite Synthesis.Metabolites · 2024Article
- Nanobioelectrocatalysis Using Human Liver Microsomes and Cytochrome P450 Bactosomes: Pyrenyl-Nanocarbon Electrodes.ACS applied bio materials · 2024Article
- In-line formation and identification of toxic reductive metabolites of aristolochic acid using electrochemistry mass spectrometry coupling.Analytical and bioanalytical chemistry · 2022Article
- Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications.The Journal of biological chemistry · 2020Review
- Electrochemiluminescence Arrays for Studies of Metabolite-related Toxicity.Electroanalysis · 2016Article
- A Cytochrome P450 3A4 Biosensor Based on Generation 4.0 PAMAM Dendrimers for the Detection of Caffeine.Biosensors · 2016Article
- Electrocatalysis by subcellular liver fractions bound to carbon nanostructures for stereoselective green drug metabolite synthesis.Chemical communications (Cambridge, England) · 2015Article
- Review
- High-throughput metabolic genotoxicity screening with a fluidic microwell chip and electrochemiluminescence.Lab on a chip · 2013Article
- Electrochemistry of mammalian cytochrome P450 2B4 indicates tunable thermodynamic parameters in surfactant films.Journal of inorganic biochemistry · 2013Article
- Paper-based electrochemiluminescent screening for genotoxic activity in the environment.Environmental science & technology · 2013Article
- Screening reactive metabolites bioactivated by multiple enzyme pathways using a multiplexed microfluidic system.The Analyst · 2013Article
- Electrochemical Activation of the Natural Catalytic Cycle of Cytochrome P450s in Human Liver Microsomes.Electroanalysis · 2012Article
- Microfluidic electrochemical array for detection of reactive metabolites formed by cytochrome P450 enzymes.Analytical chemistry · 2011Article
- Elucidating Organ-Specific Metabolic Toxicity Chemistry from Electrochemiluminescent Enzyme/DNA Arrays and Bioreactor Bead-LC-MS/MS.Chemical scienceArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Cytochrome P450s (cyt P450s) are the major oxidative enzymes in human oxidative metabolism of drugs and xenobiotic chemicals. In nature, the iron heme cyt P450s utilize oxygen and electrons delivered from NADPH by a reductase enzyme to oxidize substrates stereo- and regioselectively. Significant research has been directed toward achieving these events electrochemically. This Feature Article discusses the direct electrochemistry of cyt P450s in thin films and the utilization of such films for electrochemically driven biocatalysis. Maintaining and confirming structural integrity and catalytic activity of cyt P450s in films is an essential feature of these efforts. We highlight here our efforts to elucidate the influence of iron heme spin state and secondary structure of human cyt P450s on voltammetric and biocatalytic properties, using methodologies to quantitatively describe the dynamics of these processes in thin films. We also describe the first cyt P450/reductase films that accurately mimic the natural biocatalytic pathway and show how they can be used with voltammetry to elucidate key mechanistic features. Such bioelectronic cyt P450 systems have high value for future drug development, toxicity screening, fundamental investigations, and chemical synthesis systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.