ArticleProceedings of the National Academy of Sciences of the United States of America1990
Calcitonin gene-related peptide stimulates proliferation of human endothelial cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 294 citations in OpenAlex.
- Calcitonin gene-related peptide and pain: a systematic review.The journal of headache and pain · 2017Pooled it
- Involvement of neuronal factors in tumor angiogenesis and the shaping of the cancer microenvironment.Frontiers in immunology · 2024Review
- Research Progress in Calcitonin Gene-Related Peptide and Bone Repair.Biomolecules · 2023Review
- Calcitonin gene-related peptide: a potential protective agent in cerebral ischemia-reperfusion injury.Frontiers in neuroscience · 2023Review
- The Vascular-Dependent and -Independent Actions of Calcitonin Gene-Related Peptide in Cardiovascular Disease.Frontiers in physiology · 2022Review
- Desensitization of Capsaicin-Sensitive Afferents Accelerates Early Tumor GrowthFrontiers in oncology · 2021Article
- In Vitro Innovation of Tendon Tissue Engineering Strategies.International journal of molecular sciences · 2020Review
- Construction of calcitonin gene-related peptide-modified mesenchymal stem cells and analysis of their effects on the migration and proliferation of vascular smooth muscle cells.In vitro cellular & developmental biology. Animal · 2020Article
- Autonomic nerve dysfunction and impaired diabetic wound healing: The role of neuropeptides.Autonomic neuroscience : basic & clinical · 2020Review
- Calcitonin gene-related peptide promotes proliferation and inhibits apoptosis in endothelial progenitor cells via inhibiting MAPK signaling.Proteome science · 2018Article
- Heteroreceptors Modulating CGRP Release at Neurovascular Junction: Potential Therapeutic Implications on Some Vascular-Related Diseases.BioMed research international · 2016Review
- Calcitonin gene-related peptide in the joint: contributions to pain and inflammation.British journal of clinical pharmacology · 2015Review
- Novel roles of perivascular nerves on neovascularization.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2015Review
- Expression of calcitonin gene-related peptide in rat pulp and periodontal tissues by indirect immunofluorescence method.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2013Article
- Influence of Comorbidities: Neuropathy, Vasculopathy, and Diabetes on Healing Response Quality.Advances in wound care · 2013Review
- Neuronal regulation of tendon homoeostasis.International journal of experimental pathology · 2013Review
- Endosomal proteolysis regulates calcitonin gene-related peptide responses in mesenteric arteries.British journal of pharmacology · 2012Article
- Differentiation state determines neural effects on microvascular endothelial cells.Experimental cell research · 2012Article
- A role for the sensory neuropeptide calcitonin gene-related peptide in endothelial cell proliferation in vivo.British journal of pharmacology · 2012Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of the vasoactive perivascular neuropeptides calcitonin gene-related peptide (CGRP), neurokinin A (NKA), neuropeptide Y (NPY), and vasoactive intestinal polypeptide (VIP) on proliferation of cultured human umbilical vein endothelial cells (HUVECs) were investigated. CGRP was shown to increase both cell number and DNA synthesis, whereas NKA, NPY, and VIP were ineffective. 125I-labeled CGRP was shown to bind to HUVECs and this binding was displaced by addition of unlabeled CGRP, suggesting the existence of specific CGRP receptors. The effect of CGRP on formation of adenosine 3',5'-cyclic monophosphate (cAMP) and inositol phosphates (InsP), two intracellular messengers known to be involved in regulation of cell proliferation, was investigated. CGRP stimulated cAMP formation but was without effect on the formation of InsP. Proliferation, as well as cAMP formation, was also stimulated by cholera toxin. Basic fibroblast growth factor stimulated growth without affecting cAMP or InsP formation, whereas thrombin, which increased InsP formation, did not stimulate proliferation. We thus suggest that CGRP may act as a local factor stimulating proliferation of endothelial cells; that the mechanism of action is associated with cAMP formation; and that this effect of CGRP may be important for formation of new vessels during physiological and pathophysiological events such as ischemia, inflammation, and wound healing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.