ArticlePloS one2011
Proliferative hypothalamic neurospheres express NPY, AGRP, POMC, CART and Orexin-A and differentiate to functional neurons.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 45 citations in OpenAlex.
- An optimized method for directed differentiation of hypothalamic neural stem cells in a 3D culture system.Scientific reports · 2025Article
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- Activation of GPR40 induces hypothalamic neurogenesis through p38- and BDNF-dependent mechanisms.Scientific reports · 2020Article
- An Efficient Method for Generating Murine Hypothalamic Neurospheres for the Study of Regional Neural Progenitor Biology.Endocrinology · 2020Article
- Environment and Gene Association With Obesity and Their Impact on Neurodegenerative and Neurodevelopmental Diseases.Frontiers in neuroscience · 2020Review
- Inter-tissue communication in cancer cachexia.Nature reviews. Endocrinology · 2018Review
- In vivo maternal and in vitro BPA exposure effects on hypothalamic neurogenesis and appetite regulators.Environmental research · 2018Article
- Hypocretins, Neural Systems, Physiology, and Psychiatric Disorders.Current psychiatry reports · 2016Review
- Research Resource: The Dexamethasone Transcriptome in Hypothalamic Embryonic Neural Stem Cells.Molecular endocrinology (Baltimore, Md.) · 2016Article
- The Role of Hypothalamic Neuropeptides in Neurogenesis and Neuritogenesis.Neural plasticity · 2016Review
- Noncanonical Sites of Adult Neurogenesis in the Mammalian Brain.Cold Spring Harbor perspectives in biology · 2015Review
- Zebrafish adult-derived hypothalamic neurospheres generate gonadotropin-releasing hormone (GnRH) neurons.Biology open · 2015Article
- Neuropeptide Y stimulates autophagy in hypothalamic neurons.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Nonmuscle Tissues Contribution to Cancer Cachexia.Mediators of inflammation · 2015Review
- Effects of black adzuki bean (Vigna angularis, Geomguseul) extract on body composition and hypothalamic neuropeptide expression in rats fed a high-fat diet.Food & nutrition research · 2015Article
- Development of circadian oscillators in neurosphere cultures during adult neurogenesis.PloS one · 2015Article
- Major epigenetic development distinguishing neuronal and non-neuronal cells occurs postnatally in the murine hypothalamus.Human molecular genetics · 2014Article
- Fluoxetine induces proliferation and inhibits differentiation of hypothalamic neuroprogenitor cells in vitro.PloS one · 2014Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Some pathological conditions with feeding pattern alterations, including obesity and Huntington disease (HD) are associated with hypothalamic dysfunction and neuronal cell death. Additionally, the hypothalamus is a neurogenic region with the constitutive capacity to generate new cells of neuronal lineage, in adult rodents. The aim of the present work was to evaluate the expression of feeding-related neuropeptides in hypothalamic progenitor cells and their capacity to differentiate to functional neurons which have been described to be affected by hypothalamic dysfunction. Our study shows that hypothalamic progenitor cells from rat embryos grow as floating neurospheres and express the feeding-related neuropeptides Neuropeptide Y (NPY), Agouti-related Protein (AGRP), Pro-OpioMelanocortin (POMC), Cocaine-and-Amphetamine Responsive Transcript (CART) and Orexin-A/Hypocretin-1. Moreover the relative mRNA expression of NPY and POMC increases during the expansion of hypothalamic neurospheres in proliferative conditions.Mature neurons were obtained from the differentiation of hypothalamic progenitor cells including NPY, AGRP, POMC, CART and Orexin-A positive neurons. Furthermore the relative mRNA expression of NPY, CART and Orexin-A increases after the differentiation of hypothalamic neurospheres. Similarly to the adult hypothalamic neurons the neurospheres-derived neurons express the glutamate transporter EAAT3. The orexigenic and anorexigenic phenotype of these neurons was identified by functional response to ghrelin and leptin hormones, respectively. This work demonstrates the presence of appetite-related neuropeptides in hypothalamic progenitor cells and neurons obtained from the differentiation of hypothalamic neurospheres, including the neuronal phenotypes that have been described by others as being affected by hypothalamic neurodegeneration. These in vitro models can be used to study hypothalamic progenitor cells aiming a therapeutic intervention to mitigate feeding dysfunction that are associated with hypothalamic neurodegeneration.
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