ArticlePloS one2011
Nrt1 and Tna1-independent export of NAD+ precursor vitamins promotes NAD+ homeostasis and allows engineering of vitamin production.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Horizontal gene transfer in Saccharomyces cerevisiae and other Saccharomycotina yeasts: a review.World journal of microbiology & biotechnology · 2026Review
- Nicotinamide Riboside, a Promising Vitamin BMolecules (Basel, Switzerland) · 2023Review
- Molecular Response ofJournal of fungi (Basel, Switzerland) · 2023Article
- Emerging Role of Nicotinamide Riboside in Health and Diseases.Nutrients · 2022Review
- The role of NAD and NAD precursors on longevity and lifespan modulation in the budding yeast, Saccharomyces cerevisiae.Biogerontology · 2022Review
- Microbial Cell Factories for Green Production of Vitamins.Frontiers in bioengineering and biotechnology · 2021Review
- Nicotinamide, Nicotinamide Riboside and Nicotinic Acid-Emerging Roles in Replicative and Chronological Aging in Yeast.Biomolecules · 2020Review
- Endogenous nicotinamide riboside metabolism protects against diet-induced liver damage.Nature communications · 2019Article
- The emergence of the nicotinamide riboside kinases in the regulation of NAD+ metabolismJournal of molecular endocrinology · 2018Review
- A functional link between NADThe Journal of biological chemistry · 2018Article
- Generation, Release, and Uptake of the NAD Precursor Nicotinic Acid Riboside by Human Cells.The Journal of biological chemistry · 2015Article
- Calorie restriction-mediated replicative lifespan extension in yeast is non-cell autonomous.PLoS biology · 2015Article
- Uptake and accumulation of B-group vitamers in Saccharomyces cerevisiae in ethanol-stat fed-batch culture.World journal of microbiology & biotechnology · 2014Article
- An NAD(+) biosynthetic pathway enzyme functions cell non-autonomously in C. elegans development.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- Yeast sirtuins and the regulation of aging.FEMS yeast research · 2014Review
- Article
- Nutrient salvaging and metabolism by the intracellular pathogen Legionella pneumophila.Frontiers in cellular and infection microbiology · 2014Review
- Nicotinic acid modulates Legionella pneumophila gene expression and induces virulence traits.Infection and immunity · 2013Article
- The dynamic regulation of NAD metabolism in mitochondria.Trends in endocrinology and metabolism: TEM · 2012Article
- Novel synthetic route to the C-nucleoside, 2-deoxy benzamide riboside.Bioorganic & medicinal chemistry letters · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
NAD(+) is both a co-enzyme for hydride transfer enzymes and a substrate of sirtuins and other NAD(+) consuming enzymes. NAD(+) biosynthesis is required for two different regimens that extend lifespan in yeast. NAD(+) is synthesized from tryptophan and the three vitamin precursors of NAD(+): nicotinic acid, nicotinamide and nicotinamide riboside. Supplementation of yeast cells with NAD(+) precursors increases intracellular NAD(+) levels and extends replicative lifespan. Here we show that both nicotinamide riboside and nicotinic acid are not only vitamins but are also exported metabolites. We found that the deletion of the nicotinamide riboside transporter, Nrt1, leads to increased export of nicotinamide riboside. This discovery was exploited to engineer a strain to produce high levels of extracellular nicotinamide riboside, which was recovered in purified form. We further demonstrate that extracellular nicotinamide is readily converted to extracellular nicotinic acid in a manner that requires intracellular nicotinamidase activity. Like nicotinamide riboside, export of nicotinic acid is elevated by the deletion of the nicotinic acid transporter, Tna1. The data indicate that NAD(+) metabolism has a critical extracellular element in the yeast system and suggest that cells regulate intracellular NAD(+) metabolism by balancing import and export of NAD(+) precursor vitamins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.