ArticleInternational journal of nanomedicine2011
Plasmid-encapsulated polyethylene glycol-grafted polyethylenimine nanoparticles for gene delivery into rat mesenchymal stem cells.
Article in International journal of nanomedicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
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- Graphene Nanoplatelets Modified with Amino-Groups by Ultrasonic Radiation of Variable Frequency for Potential Adsorption of Uremic Toxins.Nanomaterials (Basel, Switzerland) · 2019Article
- The Role of Gene Therapy in Cartilage Repair.The archives of bone and joint surgery · 2019Review
- Stem cells technology: a powerful tool behind new brain treatments.Drug delivery and translational research · 2018Review
- Smart pH-sensitive nanoassemblies with cleavable PEGylation for tumor targeted drug delivery.Scientific reports · 2017Article
- Customized biomaterials to augment chondrocyte gene therapy.Acta biomaterialia · 2017Article
- Magnetic Nanoparticle-Based Upregulation of B-Cell Lymphoma 2 Enhances Bone Regeneration.Stem cells translational medicine · 2017Article
- Efficient delivery of C/EBP beta gene into human mesenchymal stem cells via polyethylenimine-coated gold nanoparticles enhances adipogenic differentiation.Scientific reports · 2016Article
- Engineering mesenchymal stem cells for regenerative medicine and drug delivery.Methods (San Diego, Calif.) · 2015Review
- Adenovirus-mediated RAR-β over-expression enhances ATRA-induced neuronal differentiation of rat mesenchymal stem cells.Archives of medical science : AMS · 2013Article
- Mesenchymal stem cell-based therapy.Molecular pharmaceutics · 2013Review
- Clinical significance of metallothioneins in cell therapy and nanomedicine.International journal of nanomedicine · 2013Review
- Cationic lipid-coated PEI/DNA polyplexes with improved efficiency and reduced cytotoxicity for gene delivery into mesenchymal stem cells.International journal of nanomedicine · 2012Article
- Functionalized nanostructures with application in regenerative medicine.International journal of molecular sciences · 2012Review
- Influence of nanomaterials on stem cell differentiation: designing an appropriate nanobiointerface.International journal of nanomedicine · 2012Review
- Generation of a focused poly(amino ether) library: polymer-mediated transgene delivery and gold-nanorod based theranostic systems.Theranostics · 2012Article
- Amphiphilic graft copolymer based on poly(styrene-co-maleic anhydride) with low molecular weight polyethylenimine for efficient gene delivery.International journal of nanomedicine · 2012Article
- Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism.International journal of nanomedicine · 2011Article
- Receptor-mediated gene delivery by folic acid-modified stearic acid-grafted chitosan micelles.International journal of nanomedicine · 2011Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMesenchymal stem cell transplantation is a promising method in regenerative medicine. Gene-modified mesenchymal stem cells possess superior characteristics of specific tissue differentiation, resistance to apoptosis, and directional migration. Viral vectors have the disadvantages of potential immunogenicity, carcinogenicity, and complicated synthetic procedures. Polyethylene glycol-grafted polyethylenimine (PEG-PEI) holds promise in gene delivery because of easy preparation and potentially targeting modification.
methodsA PEG8k-PEI25k graft copolymer was synthesized. Agarose gel retardation assay and dynamic light scattering were used to determine the properties of the nanoparticles. MTT reduction, wound and healing, and differentiation assays were used to test the cytobiological characteristics of rat mesenchymal stem cells, fluorescence microscopy and flow cytometry were used to determine transfection efficiency, and atomic force microscopy was used to evaluate the interaction between PEG-PEI/plasmid nanoparticles and mesenchymal stem cells.
resultsAfter incubation with the copolymer, the bionomics of mesenchymal stem cells showed no significant change. The mesenchymal stem cells still maintained high viability, resettled the wound area, and differentiated into adipocytes and osteoblasts. The PEG-PEI completely packed plasmid and condensed plasmid into stable nanoparticles of 100-150 nm diameter. After optimizing the N/P ratio, the PEG-PEI/plasmid microcapsules delivered plasmid into mesenchymal stem cells and obtained an optimum transfection efficiency of 15%-21%, which was higher than for cationic liposomes.
conclusionThese data indicate that PEG-PEI is a valid gene delivery agent and has better transfection efficiency than cationic liposomes in mesenchymal stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.