ArticleNucleic acids research2011
Identification of a DNA-binding site for the transcription factor Haa1, required for Saccharomyces cerevisiae response to acetic acid stress.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed.
- Mining Genetically Encoded Biosensors from Filamentous Fungi.Journal of fungi (Basel, Switzerland) · 2026Review
- Transcriptional response of Saccharomyces cerevisiae to lactic acid enantiomers.Applied microbiology and biotechnology · 2024Article
- Protein Kinase A Negatively Regulates the Acetic Acid Stress Response inMicroorganisms · 2024Article
- General mechanisms of weak acid-tolerance and current strategies for the development of tolerant yeasts.World journal of microbiology & biotechnology · 2023Review
- The Hrk1 kinase is a determinant of acetic acid tolerance in yeast by modulating HMicrobial cell (Graz, Austria) · 2023Article
- A New Mechanism of Carbon Metabolism and Acetic Acid Balance Regulated by CcpA.Microorganisms · 2023Article
- Identification of acetic acid sensitive strains through biosensor-based screening of a Saccharomyces cerevisiae CRISPRi library.Microbial cell factories · 2022Article
- Combined roles of exporters in acetic acid tolerance in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2022Article
- Whole-Genome Transformation of Yeast Promotes Rare Host Mutations with a Single Causative SNP Enhancing Acetic Acid Tolerance.Molecular and cellular biology · 2022Article
- Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains.Biotechnology for biofuels and bioproducts · 2022Review
- The cell wall and the response and tolerance to stresses of biotechnological relevance in yeasts.Frontiers in microbiology · 2022Review
- The NPR/Hal family of protein kinases in yeasts: biological role, phylogeny and regulation under environmental challenges.Computational and structural biotechnology journal · 2022Review
- Identification of Kic1p and Cdc42p as Novel Targets to Engineer Yeast Acetic Acid Stress Tolerance.Frontiers in bioengineering and biotechnology · 2022Article
- Development of an Haa1-based biosensor for acetic acid sensing in Saccharomyces cerevisiae.FEMS yeast research · 2021Article
- From a genome assembly to full regulatory network prediction: the case study of Rhodotorula toruloides putative Haa1-regulon.BMC bioinformatics · 2021Article
- A fluorescence-based yeast sensor for monitoring acetic acid.Engineering in life sciences · 2021Article
- Valorisation of pectin-rich agro-industrial residues by yeasts: potential and challenges.Applied microbiology and biotechnology · 2020Review
- YEASTRACT+: a portal for cross-species comparative genomics of transcription regulation in yeasts.Nucleic acids research · 2020Article
- Carbon Catabolite Repression in Yeast is Not Limited to Glucose.Scientific reports · 2019Article
- Activation of Haa1 and War1 transcription factors by differential binding of weak acid anions in Saccharomyces cerevisiae.Nucleic acids research · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
The transcription factor Haa1 is the main player in reprogramming yeast genomic expression in response to acetic acid stress. Mapping of the promoter region of one of the Haa1-activated genes, TPO3, allowed the identification of an acetic acid responsive element (ACRE) to which Haa1 binds in vivo. The in silico analysis of the promoter regions of the genes of the Haa1-regulon led to the identification of an Haa1-responsive element (HRE) 5'-GNN(G/C)(A/C)(A/G)G(A/G/C)G-3'. Using surface plasmon resonance experiments and electrophoretic mobility shift assays it is demonstrated that Haa1 interacts with high affinity (K(D) of 2 nM) with the HRE motif present in the ACRE region of TPO3 promoter. No significant interaction was found between Haa1 and HRE motifs having adenine nucleotides at positions 6 and 8 (K(D) of 396 and 6780 nM, respectively) suggesting that Haa1p does not recognize these motifs in vivo. A lower affinity of Haa1 toward HRE motifs having mutations in the guanine nucleotides at position 7 and 9 (K(D) of 21 and 119 nM, respectively) was also observed. Altogether, the results obtained indicate that the minimal functional binding site of Haa1 is 5'-(G/C)(A/C)GG(G/C)G-3'. The Haa1-dependent transcriptional regulatory network active in yeast response to acetic acid stress is proposed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.