ArticlePlant cell reports2011
Agrobacterium-mediated transformation of finger millet (Eleusine coracana (L.) Gaertn.) using shoot apex explants.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 107 citations in OpenAlex.
- Accelerating iron biofortification in millets: progress, challenges, and future prospects.Planta · 2026Review
- Rapid Agrobacterium-mediated transformation and high-efficiency regeneration of finger millet (Eleusine coracana) for crop improvement.Transgenic research · 2026Article
- Integrative insights into abiotic stress tolerance in finger millet (Frontiers in plant science · 2026Review
- Toward improving multiomic research in understudied cereals.Nature genetics · 2025Review
- Millets for a sustainable future.Journal of experimental botany · 2025Review
- Enhancing tiny millets through genome editing: current status and future prospects.Molecular genetics and genomics : MGG · 2025Review
- Review
- Biotechnological studies towards improvement of finger millet using multi-omics approaches.Functional & integrative genomics · 2024Review
- Milletomics: a metabolomics centered integrated omics approach toward genetic progression.Functional & integrative genomics · 2024Review
- Finger millet: a hero in the making to combat food insecurity.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Genetic engineering and genome editing technologies as catalyst for Africa's food security: the case of plant biotechnology in Nigeria.Frontiers in genome editing · 2024Review
- Review
- The potentialities of omics resources for millet improvement.Functional & integrative genomics · 2023Review
- Review
- Photosynthetic electron transport rate and root dynamics of finger millet in response toPlant signaling & behavior · 2022Article
- Beyond a reference genome: pangenomes and population genomics of underutilized and orphan crops for future food and nutrition security.The New phytologist · 2022Review
- Using plant growth-promoting microorganisms (PGPMs) to improve plant development under in vitro culture conditions.Planta · 2022Review
- Genome-editing in millets: current knowledge and future perspectives.Molecular biology reports · 2022Review
- CRISPR for accelerating genetic gains in under-utilized crops of the drylands: Progress and prospects.Frontiers in genetics · 2022Review
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new Agrobacterium-mediated transformation system was developed for finger millet using shoot apex explants. The Agrobacterium strain LBA4404 harboring binary vector pCAMBIA1301, which contained hygromycin phosphotransferase (hptII) as selectable marker gene and β-glucuronidase (GUS) as reporter gene, was used for optimization of transformation conditions. Two finger millet genotypes, GPU 45 and CO 14, were used in this study. The optimal conditions for the Agrobacterium-mediated transformation of finger millet were found to be the co-cultivation of explants obtained on the 16th day after callus induction (DACI), exposure of explants for 30 min to agrobacterial inoculum and 3 days of co-cultivation on filter paper placed on medium supplemented with 100 μM acetosyringone (AS). Addition of 100 μM L: -cysteine in the selection medium enhanced the frequency of transformation and transgenic plant recovery. Both finger millet genotypes were transformed by Agrobacterium. A frequency of 19% transient expression with 3.8% stable transformation was achieved in genotype GPU 45 using optimal conditions. Five stably transformed plants were fully characterized by Southern blot analysis. A segregation analysis was also performed in four R(1) progenies, which showed normal Mendelian pattern of transgene segregation. The inheritance of transgenes in R(1) progenies was also confirmed by Southern blot analysis. This is the first report on Agrobacterium-mediated transformation of finger millet. This study underpins the introduction of numerous agronomically important genes into the genome of finger millet in the future.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.