ArticleJournal of innate immunity2011
Importance of phagosomal functionality for growth restriction of Mycobacterium tuberculosis in primary human macrophages.
Article in Journal of innate immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 78 citations in OpenAlex.
- All-trans retinoic acid-triggered antimicrobial activity against Mycobacterium tuberculosis is dependent on NPC2.Journal of immunology (Baltimore, Md. : 1950) · 2014Trial
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- Host-Directed Therapies Based on Protease Inhibitors to ControlMicroorganisms · 2025Review
- Modulation of Cystatin F in Human Macrophages Impacts Cathepsin-Driven Killing of Multidrug-ResistantMicroorganisms · 2023Article
- Transcriptomic Analysis of Mycobacterial Infected Macrophages Reveals a High MOI Specific Type I IFN Signaling.Infection and immunity · 2023Article
- Development of Chitosan Particles Loaded with siRNA for Cystatin C to Control Intracellular Drug-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- Liposomal Delivery of Saquinavir to Macrophages Overcomes Cathepsin Blockade byInternational journal of molecular sciences · 2023Article
- Analysis of the noncoding RNA regulatory networks of H37Rv- and H37Rv△1759c-infected macrophages.Frontiers in microbiology · 2023Article
- Role of phagocyte extracellular traps duringFrontiers in microbiology · 2023Review
- Persistence ofFrontiers in cellular and infection microbiology · 2022Article
- Article
- Critical Evaluation of Different Lysosomal Labeling Methods Used to Analyze RNA Nanocarrier Trafficking in Cells.Bioconjugate chemistry · 2021Article
- Intracellular localisation of Mycobacterium tuberculosis affects efficacy of the antibiotic pyrazinamide.Nature communications · 2021Article
- The Interplay of Host Lysosomes and Intracellular Pathogens.Frontiers in cellular and infection microbiology · 2020Review
- Variations in Antimicrobial Activities of Human Monocyte-Derived Macrophage and Their Associations With Tuberculosis Clinical Manifestations.Frontiers in cellular and infection microbiology · 2020Article
- Mycobacterium tuberculosis infection of host cells in space and time.FEMS microbiology reviews · 2019Review
- Functional aspects, phenotypic heterogeneity, and tissue immune response of macrophages in infectious diseases.Infection and drug resistance · 2019Article
- Human Antimicrobial RNases Inhibit Intracellular Bacterial Growth and Induce Autophagy in Mycobacteria-Infected Macrophages.Frontiers in immunology · 2019Article
- MyD88 in Mycobacterium tuberculosis infection.Medical microbiology and immunology · 2017Review
- Molecular basis of mycobacterial survival in macrophages.Cellular and molecular life sciences : CMLS · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The best characterized survival mechanism of Mycobacterium tuberculosis inside the macrophage is the inhibition of phagosomal maturation. Phagosomal maturation involves several steps including fusion with lysosomes and acidification. However, it has not been elucidated which components of phagosomal maturation correlate with growth restriction of virulent mycobacteria in human macrophages, and we aimed to study this. We infected human monocyte-derived macrophages with M. tuberculosis and assessed bacterial replication, translocation of CD63 to the phagosome, and phagosomal acidification. We found that unstimulated human macrophages were able to control infection with M. tuberculosis upon inoculation at a low multiplicity of infection (MOI) of 1, but not at a high MOI of 10. The low MOI resulted in a macrophage-controlled balance between host cells and bacteria. Both H37Rv and H37Ra infection, at high and low MOI, led to equally ineffective translocation of CD63 to the phagosome. On the other hand, acidification of mycobacterial phagosomes was more efficient at MOI 1 than 10 with both mycobacterial strains, consistent with a direct or indirect role for phagosomal acidification in restricting M. tuberculosis growth. Furthermore, inhibition of the vacuolar H(+)-ATPase as well as of cathepsin D led to enhanced mycobacterial replication inside the macrophage. This again shows the importance of phagosomal acidification for control of mycobacterial growth, through the activation of lysosomal hydrolases. We conclude that acidification and related functional aspects of the mature phagosome are important factors for restriction of M. tuberculosis replication in human macrophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.