ArticleProceedings of the National Academy of Sciences of the United States of America2011
Cell-free sorting of peroxisomal membrane proteins from the endoplasmic reticulum.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
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Who cites it
53 citing papers in PubMed, 96 citations in OpenAlex.
- Peroxisome biogenesis and inter-organelle communication: an indispensable role for Pex11 and Pex30 family proteins in yeast.Current genetics · 2022Review
- Sharing the wealth: The versatility of proteins targeted to peroxisomes and other organelles.Frontiers in cell and developmental biology · 2022Review
- Hypothyroidism Intensifies Both Canonic and theCells · 2021Article
- Glycosome heterogeneity in kinetoplastids.Biochemical Society transactions · 2021Review
- Peroxisomal Membrane Contact Sites in Yeasts.Frontiers in cell and developmental biology · 2021Review
- Peroxisome prognostications: Exploring the birth, life, and death of an organelle.The Journal of cell biology · 2020Review
- Peroxisome Maintenance Depends on De Novo Peroxisome Formation in Yeast Mutants Defective in Peroxisome Fission and Inheritance.International journal of molecular sciences · 2019Article
- Regulatory Roles of Peroxisomal Metabolic Pathways Involved in Musk Secretion in Muskrats.The Journal of membrane biology · 2019Article
- Peroxisome biogenesis, membrane contact sites, and quality control.EMBO reports · 2019Review
- Lipid Droplet and Peroxisome Biogenesis: Do They Go Hand-in-Hand?Frontiers in cell and developmental biology · 2019Review
- Seipin and the membrane-shaping protein Pex30 cooperate in organelle budding from the endoplasmic reticulum.Nature communications · 2018Article
- ESCRT-III is required for scissioning new peroxisomes from the endoplasmic reticulum.The Journal of cell biology · 2018Article
- Mind the Organelle Gap - Peroxisome Contact Sites in Disease.Trends in biochemical sciences · 2018Review
- A New Yeast Peroxin, Pex36, a Functional Homolog of Mammalian PEX16, Functions in the ER-to-Peroxisome Traffic of Peroxisomal Membrane Proteins.Journal of molecular biology · 2017Article
- Saccharomyces cerevisiae cells lacking Pex3 contain membrane vesicles that harbor a subset of peroxisomal membrane proteins.Biochimica et biophysica acta. Molecular cell research · 2017Article
- Organelle biogenesis in the endoplasmic reticulum.Nature cell biology · 2017Review
- Functional regions of the peroxin Pex19 necessary for peroxisome biogenesis.The Journal of biological chemistry · 2017Article
- Glycosome biogenesis in trypanosomes and the de novo dilemma.PLoS neglected tropical diseases · 2017Review
- Article
- VAPs and ACBD5 tether peroxisomes to the ER for peroxisome maintenance and lipid homeostasis.The Journal of cell biology · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Several yeast and mammalian peroxisomal membrane proteins (PMPs) are delivered to peroxisomes via the endoplasmic reticulum (ER). Fluorescence microscopy showed a focused assembly of PMPs in a specialized domain of the ER, referred to as the preperoxisomal ER. It is proposed that preperoxisomal vesicles containing PMPs bud from this domain to either fuse with preexisting peroxisomes or to mature into functional peroxisomes by uptake of peroxisomal membrane and matrix proteins. However, such vesicular entities are not identified nor are the biochemical requirements for the budding process known. We developed an in vitro cell-free ER-budding assay using Pichia pastoris and followed two endogenous PMPs, Pex11p and Pex3p during their ER exit. Both the PMPs were copackaged in the ER-budded vesicles that float on a Nycodenz gradient. PMP budding from the ER was dependent on ATP, temperature, cytosol, and Pex19p and generated preperoxisomal vesicles with an incomplete complement of PMPs. Surprisingly, Pex11p budding was independent of Pex3p; however, the budded vesicles were devoid of most of the PMPs otherwise present in the wild-type vesicles and might represent peroxisomal remnants. Our findings provide a biochemical platform to uncover the mechanism of PMP budding from the ER.
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Registered trials
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