ArticleMolecular and cellular biology2011
An extended domain of Kcnq1ot1 silencing revealed by an imprinted fluorescent reporter.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- The structure of the TH/INS locus and the parental allele expressed are not conserved between mammals.Heredity · 2024Article
- Transcription factor ASCL2 is required for development of the glycogen trophoblast cell lineage.PLoS genetics · 2018Article
- Nucleoporin 107, 62 and 153 mediate Kcnq1ot1 imprinted domain regulation in extraembryonic endoderm stem cells.Nature communications · 2018Article
- Visualizing Changes in Cdkn1c Expression Links Early-Life Adversity to Imprint Mis-regulation in Adults.Cell reports · 2017Article
- Long non-coding RNAs: Mechanism of action and functional utility.Non-coding RNA research · 2016Review
- The functional role of long non-coding RNAs and epigenetics.Biological procedures online · 2014Review
- Chromatin structure, epigenetic mechanisms and long-range interactions in the human insulin locus.Diabetes, obesity & metabolism · 2012Review
- The placental imprintome and imprinted gene function in the trophoblast glycogen cell lineage.Reproductive biomedicine online · 2012Review
- Zinc finger protein ZFP57 requires its co-factor to recruit DNA methyltransferases and maintains DNA methylation imprint in embryonic stem cells via its transcriptional repression domain.The Journal of biological chemistry · 2012Article
- Depletion of Kcnq1ot1 non-coding RNA does not affect imprinting maintenance in stem cells.Development (Cambridge, England) · 2011Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The distal region of mouse chromosome 7 contains two imprinted domains separated by a relatively gene-poor interval. We have previously described a transgenic mouse line called Tel7KI, which contains a green fluorescent protein (GFP) reporter inserted 2.6 kb upstream of the Ins2 gene at the proximal end of this interval. The GFP reporter from Tel7KI is imprinted and maternally expressed in postimplantation embryos. Here, we present evidence that the distal imprinting center, KvDMR1 (IC2), is responsible for the paternal silencing of Tel7KI. First, we show that Tel7KI is silenced when the noncoding RNA Kcnq1ot1 is biallelically expressed due to absence of maternal DNA methylation at IC2. Second, we use an embryonic stem (ES) cell differentiation assay to examine the effect of an IC2 deletion in cis to Tel7KI and show that it impairs the ability of the paternal transmission Tel7KI ES cells to silence GFP. These results suggested that Kcnq1ot1 silencing extends nearly 300 kb further than previously reported and led us to examine other transcripts between IC1 and IC2. We found that splice variants of Th and Ins2 are imprinted, maternally expressed, and regulated by IC2, showing that the silencing domain uncovered by our transgenic line also affects endogenous transcripts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.