ArticleInfection and immunity2011
Genomic and surface proteomic analysis of the canine pathogen Staphylococcus pseudintermedius reveals proteins that mediate adherence to the extracellular matrix.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 76 citations in OpenAlex.
- Investigations of Procalcitonin, Interleukin-8 and Defensin-β in Dogs with Superficial and Deep Pyoderma.Veterinary sciences · 2026Article
- Staphylococcal internalization into osteoblasts: a partially conserved mechanism across the genus.mBio · 2026Article
- Comparative phylogenetic, antimicrobial resistance, and clinical characterization of human spondylodiscitis-associatedFrontiers in microbiology · 2026Article
- The dynamic interplay betweenFrontiers in cellular and infection microbiology · 2026Review
- Investigation on biofilm composition and virulence traits of S. pseudintermedius isolated from infected and colonized dogs.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Isolation ofMicroorganisms · 2024Article
- Virulence Mechanisms of Staphylococcal Animal Pathogens.International journal of molecular sciences · 2023Review
- Human Colonization and Infection byMicroorganisms · 2023Review
- Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies.PloS one · 2023Article
- Strategies to prevent, curb and eliminate biofilm formation based on the characteristics of various periods in one biofilm life cycle.Frontiers in cellular and infection microbiology · 2022Review
- From canines to humans: Clinical importance of Staphylococcus pseudintermedius.PLoS pathogens · 2021Article
- Absence of Host-Specific Genes in Canine and HumanAntibiotics (Basel, Switzerland) · 2021Article
- Searching for the Secret of Stickiness: How Biofilms Adhere to Surfaces.Frontiers in microbiology · 2021Review
- Article
- Virulence factors in coagulase-positive staphylococci of veterinary interest other thanThe veterinary quarterly · 2020Review
- Staphylococcal Protein A (mSphere · 2020Article
- Force-clamp spectroscopy identifies a catch bond mechanism in a Gram-positive pathogen.Nature communications · 2020Article
- Article
- Identification and Characterization of Staphylococcus delphini Internalization Pathway in Nonprofessional Phagocytic Cells.Infection and immunity · 2020Article
- The Role of Gram-Positive Surface Proteins in Bacterial Niche- and Host-Specialization.Frontiers in microbiology · 2020Review
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
Abstract
Cell wall-associated (CWA) proteins made by Gram-positive pathogens play a fundamental role in pathogenesis. Staphylococcus pseudintermedius is a major animal pathogen responsible for the canine skin disease bacterial pyoderma. Here, we describe the bioinformatic analysis of the family of 18 predicted CWA proteins encoded in the genome of S. pseudintermedius strain ED99 and determine their distribution among a phylogenetically diverse panel of S. pseudintermedius clinical isolates and closely related species of the Staphylococcus intermedius group. In parallel, we employed a proteomic approach to identify proteins presented on the surface of strain ED99 in vitro, revealing a total of 60 surface-localized proteins in one or more phases of growth, including 6 of the 18 genome-predicted CWA proteins. Based on these analyses, we selected two CWA proteins (SpsD and SpsL) encoded by all strains examined and investigated their capacity to mediate adherence to extracellular matrix proteins. We discovered that SpsD and SpsL mediated binding of a heterologous host, Lactococcus lactis, to fibrinogen and fibronectin and that SpsD mediated binding to cytokeratin 10, a major constituent of mammalian skin. Of note, the interaction with fibrinogen was host-species dependent, suggestive of a role for SpsD and SpsL in the host tropism of S. pseudintermedius. Finally, we identified IgG specific for SpsD and SpsL in sera from dogs with bacterial pyoderma, implying that both proteins are expressed during infection. The combined genomic and proteomic approach employed in the current study has revealed novel host-pathogen interactions which represent candidate therapeutic targets for the control of bacterial pyoderma.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.