ArticleBiomaterials2011
Microscale mechanisms of agarose-induced disruption of collagen remodeling.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 59 citations in OpenAlex.
- Piezo1 regulates remodeling of skin-derived extracellular matrix by embedded umbilical cord mesenchymal stem cells in a stiffness-dependent fashion.Materials today. Bio · 2026Article
- A novel agarose-free, standardized generation and versatile ECM characterization of decellularized scaffolds from normal and fibrotic human lung tissue.Frontiers in bioengineering and biotechnology · 2026Article
- Characterization of Composite Agarose-Collagen Hydrogels for Chondrocyte Culture.Annals of biomedical engineering · 2025Article
- Surgical Site-Released Tissue Is Potent to Generate Bone onto TCP and PCL-TCP Scaffolds In Vitro.International journal of molecular sciences · 2023Article
- Injectable three-dimensional tumor microenvironments to study mechanobiology in ovarian cancer.Acta biomaterialia · 2022Article
- Addition of collagen type I in agarose created a dose-dependent effect on matrix production in engineered cartilage.Regenerative biomaterials · 2022Article
- Evaluation of Therapeutic Collagen-Based Biomaterials in the Infarcted Mouse Heart by Extracellular Matrix Targeted MALDI Imaging Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2021Article
- Getting the big picture of cell-matrix interactions: High-throughput biomaterial platforms and systems-level measurements.Current opinion in solid state & materials science · 2020Article
- Characterization of Tissue Engineered Endothelial Cell Networks in Composite Collagen-Agarose Hydrogels.Gels (Basel, Switzerland) · 2020Article
- Fluid shear stress stimulates breast cancer cells to display invasive and chemoresistant phenotypes while upregulating PLAU in a 3D bioreactor.Biotechnology and bioengineering · 2019Article
- Dissecting and rebuilding the glioblastoma microenvironment with engineered materials.Nature reviews. Materials · 2019Article
- Biofabrication of phenotypic pulmonary fibrosis assays.Biofabrication · 2019Review
- Effects of Culture Dimensions on Maintenance of Porcine Inner Cell Mass-Derived Cell Self-Renewal.Molecules and cells · 2017Article
- N-terminal specific conjugation of extracellular matrix proteins to 2-pyridinecarboxaldehyde functionalized polyacrylamide hydrogels.Biomaterials · 2016Article
- Secondary ion mass spectrometry and Raman spectroscopy for tissue engineering applications.Current opinion in biotechnology · 2015Review
- Engineering strategies to mimic the glioblastoma microenvironment.Advanced drug delivery reviews · 2014Review
- Physical influences of the extracellular environment on cell migration.Nature reviews. Molecular cell biology · 2014Review
- Sequential assembly of 3D perfusable microfluidic hydrogels.Journal of materials science. Materials in medicine · 2014Article
- Three-dimensional patterning of multiple cell populations through orthogonal genetic control of cell motility.Soft matter · 2014Article
- Tuning three-dimensional collagen matrix stiffness independently of collagen concentration modulates endothelial cell behavior.Acta biomaterialia · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Cells are strongly influenced by the local structure and mechanics of the extracellular matrix (ECM). We recently showed that adding agarose to soft collagen ECMs can mechanically stiffen these hydrogels by two orders of magnitude while limiting 3D cell motility, which we speculated might derive from agarose-mediated inhibition of collagen fiber deformation and remodeling. Here, we directly address this hypothesis by investigating the effects of agarose on cell-collagen interactions at the microscale. Addition of agarose progressively restricts cell spreading, reduces stress fiber and focal adhesion assembly, and inhibits macroscopic gel compaction. While time-of-flight secondary ion mass spectrometry and scanning electron microscopy fail to reveal agarose-induced alterations in collagen ligand presentation, the latter modality shows that agarose strongly impairs cell-directed assembly of large collagen bundles. Agarose-mediated inhibition of cell spreading and cytoarchitecture can be rescued by β-agarase digestion or by covalently crosslinking the matrix with glutaraldehyde. Based on these results, we argue that cell spreading and motility on collagen requires local matrix stiffening, which can be achieved via cell-mediated fiber remodeling or by chemically crosslinking the fibers. These findings provide new mechanistic insights into the regulatory function of agarose and bear general implications for cell adhesion and motility in fibrous ECMs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.