ArticleMicrobial pathogenesis2011
Tn5AraOut mutagenesis for the identification of Yersinia pestis genes involved in resistance towards cationic antimicrobial peptides.
Article in Microbial pathogenesis, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Iron Transport and Metabolism in Escherichia, Shigella, and Salmonella.EcoSal Plus · 2021Article
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- The FeoC [4Fe-4S] Cluster Is Redox-Active and Rapidly Oxygen-Sensitive.Biochemistry · 2019Article
- Antimicrobial Peptide Resistance Genes in the Plant Pathogen Dickeya dadantii.Applied and environmental microbiology · 2016Article
- Lipopolysaccharide Biosynthesis Genes of Yersinia pseudotuberculosis Promote Resistance to Antimicrobial Chemokines.PloS one · 2016Article
- The role of transition metal transporters for iron, zinc, manganese, and copper in the pathogenesis of Yersinia pestis.Metallomics : integrated biometal science · 2015Review
- FeoA and FeoC are essential components of the Vibrio cholerae ferrous iron uptake system, and FeoC interacts with FeoB.Journal of bacteriology · 2013Article
- Polymyxin resistance of Pseudomonas aeruginosa phoQ mutants is dependent on additional two-component regulatory systems.Antimicrobial agents and chemotherapy · 2013Article
- Iron homeostasis in theAdvances in botanical research · 2013Article
- The Yfe and Feo transporters are involved in microaerobic growth and virulence of Yersinia pestis in bubonic plague.Infection and immunity · 2012Article
- A transposon site hybridization screen identifies galU and wecBC as important for survival of Yersinia pestis in murine macrophages.Journal of bacteriology · 2012Article
- Cationic host defense peptides; novel antimicrobial therapeutics against Category A pathogens and emerging infections.Pathogens and global healthReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Bacterial pathogens display a variety of protection mechanisms against the inhibitory and lethal effects of host cationic antimicrobial peptides (CAMPs). To identify Yersinia pestis genes involved in CAMP resistance, libraries of DSY101 (KIM6 caf1 pla psa) minitransposon Tn5AraOut mutants were selected at 37°C for resistance to the model CAMPs polymyxin B or protamine. This approach targeted genes that needed to be repressed (null mutations) or induced (upstream P(BAD) insertions) for the detection of CAMP resistance, and predictably for improved pathogen fitness in mammalian hosts. Ten mutants demonstrated increased resistance to polymyxin B or protamine, with the mapped mutations pointing towards genes suspected to participate in modifying membrane components, genes encoding transport proteins or enzymes, or the regulator of a ferrous iron uptake system (feoC). Not all the mutants were resistant to both CAMPs used for selection. None of the polymyxin B- and only some protamine-resistant mutants, including the feoC mutant, showed increased resistance to rat bronchoalveolar lavage fluid (rBALF) known to contain cathelicidin and β-defensin 1. Thus, findings on bacterial resistance to polymyxin B or protamine don't always apply to CAMPs of the mammalian innate immune system, such as the ones in rBALF.
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