ArticleBiophysical journal2011
Fully hydrated yeast cells imaged with electron microscopy.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Imaging of Hydrated and Living Cells in Transmission Electron Microscope: Summary, Challenges, and Perspectives.ACS nano · 2025Review
- A novel method for telomere length detection in fission yeast.FEMS yeast research · 2025Article
- Liquid Phase Electron Microscopy of Bacterial Ultrastructure.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Electron videography of a lipid-protein tango.Science advances · 2024Article
- Article
- Liquid cell transmission electron microscopy and its applications.Royal Society open science · 2020Review
- LL37 InhibitsFrontiers in immunology · 2019Article
- The role of electron irradiation history in liquid cell transmission electron microscopy.Science advances · 2018Article
- High-resolution imaging of living mammalian cells bound by nanobeads-connected antibodies in a medium using scanning electron-assisted dielectric microscopy.Scientific reports · 2017Article
- Dynamic nano-imaging of label-free living cells using electron beam excitation-assisted optical microscope.Scientific reports · 2015Article
- Graphene-enabled electron microscopy and correlated super-resolution microscopy of wet cells.Nature communications · 2015Article
- Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae.Biotechnology for biofuels · 2015Article
- Correlative electron and fluorescence microscopy of magnetotactic bacteria in liquid: toward in vivo imaging.Scientific reports · 2014Article
- Fungicidal mechanisms of cathelicidins LL-37 and CATH-2 revealed by live-cell imaging.Antimicrobial agents and chemotherapy · 2014Article
- Correlative cryo-electron tomography and optical microscopy of cells.Current opinion in structural biology · 2013Review
- Development and application of STEM for the biological sciences.Ultramicroscopy · 2012Review
- Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy.Micron (Oxford, England : 1993) · 2012Article
- Electron microscopy of specimens in liquid.Nature nanotechnology · 2011Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We demonstrate electron microscopy of fully hydrated eukaryotic cells with nanometer resolution. Living Schizosaccharomyces pombe cells were loaded in a microfluidic chamber and imaged in liquid with scanning transmission electron microscopy (STEM). The native intracellular (ultra)structures of wild-type cells and three different mutants were studied without prior labeling, fixation, or staining. The STEM images revealed various intracellular components that were identified on the basis of their shape, size, location, and mass density. The maximal achieved spatial resolution in this initial study was 32 ± 8 nm, an order of magnitude better than achievable with light microscopy on pristine cells. Light-microscopy images of the same samples were correlated with the corresponding electron-microscopy images. Achieving synergy between the capabilities of light and electron microscopy, we anticipate that liquid STEM will be broadly applied to explore the ultrastructure of live cells.
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