ReviewBiophysical journal2011
Reaching the information limit in cryo-EM of biological macromolecules: experimental aspects.
Review in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed.
- Probing protein-lipid interactions by hydrogen/deuterium exchange-mass spectrometry: advances, challenges and future directions.Chemical science · 2026Review
- prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.Structure (London, England : 1993) · 2026Article
- Membrane protein structure and dynamics probed by MicroED.Biochemical Society transactions · 2026Review
- Composition-Encoded Control of Amphiphilicity and Nanoscale Rigidity in Poly(isobutyl cyanoacrylate)/Poly(butylene oxide)-ACS polymers Au · 2026Article
- Direct Measurement of Protein Pair Interaction Potential.ACS nano · 2026Article
- Liquid Phase Biological Electron Microscopy: Many Published Results and Claimed Benefits Are Fantasy, Not Fact.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2025Article
- Advancing Cryo-EM and Cryo-ET through Innovation in Sample Carriers: A Perspective.Analytical chemistry · 2025Review
- TomoScore: A Neural Network Approach for Quality Assessment of Cellular cryo-ET.Research square · 2025Article
- Structures of Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus virions reveal species-specific tegument and envelope features.Journal of virology · 2024Article
- TomoScore: A Neural Network Approach for Quality Assessment of Cellular cryo-ET.bioRxiv : the preprint server for biology · 2024Article
- Application of Silicon Nanowire Field Effect Transistor (SiNW-FET) Biosensor with High Sensitivity.Sensors (Basel, Switzerland) · 2023Review
- Cryo-EM of the injectisome and type III secretion systems.Current opinion in structural biology · 2022Review
- Perspective: Emerging strategies for determining atomic-resolution structures of macromolecular complexes within cells.Journal of structural biology · 2022Article
- Quantitative Cryo-Electron Tomography.Frontiers in molecular biosciences · 2022Review
- Astrocyte-Derived Extracellular Vesicles (ADEVs): Deciphering their Influences in Aging.Aging and disease · 2021Review
- Progress Towards CryoEM: Negative-Stain Procedures for Biological Samples.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Flexible Fitting of Small Molecules into Electron Microscopy Maps Using Molecular Dynamics Simulations with Neural Network Potentials.Journal of chemical information and modeling · 2020Article
- Developments, applications, and prospects of cryo-electron microscopy.Protein science : a publication of the Protein Society · 2020Review
- Advances in methods for atomic resolution macromolecular structure determination.F1000Research · 2020Review
- Single-particle reconstruction statistics: a diagnostic tool in solving biomolecular structures by cryo-EM.Acta crystallographica. Section F, Structural biology communications · 2019Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although cryo-electron microscopy (cryo-EM) of biological macromolecules has made important advances in the past few years, the level of current technical performance is still well below what the physics of electron scattering would allow. It should be possible, for example, to use cryo-EM to solve protein structures at atomic resolution for particle sizes well below 80 kDa, but currently this has been achieved only for particles at least 10 times larger than that. In this review, we first examine some of the reasons for this large gap in performance. We then give an overview of work that is currently in progress to 1), improve the signal/noise ratio for area detectors; 2), improve the signal transfer between the scattered electrons and the corresponding images; and 3), reduce the extent to which beam-induced movement causes a steep fall-off of signal at high resolution. In each case, there is substantial reason to think that cryo-EM can indeed be made to approach the estimated physical limits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.