ArticleBMC microbiology2011
Tools for genetic manipulation of the plant growth-promoting bacterium Azospirillum amazonense.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- Loss of the flagellar regulator FlhC changes the transcriptional response of plant-associatedISME communications · 2026Article
- Two plant-hosted whole-cell bacterial biosensors for detection of bioavailable Cr(VI).World journal of microbiology & biotechnology · 2019Article
- A subcompatible rhizobium strain reveals infection duality in Lotus.Journal of experimental botany · 2019Article
- Impact of plant-associated bacteria biosensors on plant growth in the presence of hexavalent chromium.World journal of microbiology & biotechnology · 2017Article
- Development of a high-efficient transformation system of Bacillus pumilus strain DX01 to facilitate gene isolation via gfp-tagged insertional mutagenesis and visualize bacterial colonization of rice roots.Folia microbiologica · 2013Article
- Genomic insights into the versatility of the plant growth-promoting bacterium Azospirillum amazonense.BMC genomics · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAzospirillum amazonense has potential to be used as agricultural inoculant since it promotes plant growth without causing pollution, unlike industrial fertilizers. Owing to this fact, the study of this species has gained interest. However, a detailed understanding of its genetics and physiology is limited by the absence of appropriate genetic tools for the study of this species.
resultsConjugation and electrotransformation methods were established utilizing vectors with broad host-replication origins (pVS1 and pBBR1). Two genes of interest--glnK and glnB, encoding PII regulatory proteins--were isolated. Furthermore, glnK-specific A. amazonense mutants were generated utilizing the pK19MOBSACB vector system. Finally, a promoter analysis protocol based on fluorescent protein expression was optimized to aid genetic regulation studies on this bacterium.
conclusionIn this work, genetic tools that can support the study of A. amazonense were described. These methods could provide a better understanding of the genetic mechanisms of this species that underlie its plant growth promotion.
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