ReviewIntensive care medicine2011
Will polymerase chain reaction (PCR)-based diagnostics improve outcome in septic patients? A clinical view.
Review in Intensive care medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04178382 (Effect of PCR-CRISPR/Cas12a on the Early Anti-infective Schemes in Patients With Open Air Pneumonia), which is not on this map. Cited by 35 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effect of PCR-CRISPR/Cas12a on the Early Anti-infective Schemes in Patients With Open Air Pneumonia
Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.
- Volatile metabolites of pathogens: a systematic review.PLoS pathogens · 2013Pooled it
- Impact of multiplex PCR on antimicrobial treatment in febrile neutropenia: a randomized controlled study.Medical microbiology and immunology · 2015Trial
- Diagnostic accuracy of SeptiFast multi-pathogen real-time PCR in the setting of suspected healthcare-associated bloodstream infection.Intensive care medicine · 2015Trial
- Trial
- Dynamic Profiling of Lipoteichoic Acid (LTA) and/or Lipopolysaccharide (LPS) Positive Extracellular Vesicles in Plasma as Diagnostic and Prognostic Biomarkers for Bacterial Infection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Electrochemical Aptamer-Based Biosensors for Sepsis Diagnosis: Recent Advances, Challenges, and Future Perspectives (2020-2025).Biosensors · 2025Review
- Epidemiology of positive bacterial cultures and the coverage gaps in multiplex PCR diagnostics: a single-center retrospective study.Journal of clinical microbiology · 2025Article
- Association of full blood count findings with risk of mortality in children with Klebsiella pneumoniae bloodstream infection at a south african children's hospital.BMC pediatrics · 2023Article
- CRISPR-Cas based virus detection: Recent advances and perspectives.Biosensors & bioelectronics · 2021Review
- 3× multiplexed detection of antibiotic resistant plasmids with single molecule sensitivity.Lab on a chip · 2020Article
- Bacterial sepsis : Diagnostics and calculated antibiotic therapy.Der Anaesthesist · 2019Review
- Sequence-specific sepsis-related DNA capture and fluorescent labeling in monoliths prepared by single-step photopolymerization in microfluidic devices.Journal of chromatography. A · 2018Article
- Bacterial DNA patterns identified using paired-end Illumina sequencing of 16S rRNA genes from whole blood samples of septic patients in the emergency room and intensive care unit.BMC microbiology · 2018Article
- The threat of carbapenem-resistant gram-negative bacteria in a Middle East region.Infection and drug resistance · 2018Review
- [Bacterial sepsis : Diagnostics and calculated antibiotic therapy].Der Anaesthesist · 2017Review
- [Antibiotic Stewardship 2.0. Individualization of therapy].Der Internist · 2017Review
- Implementation of short incubation MALDI-TOF MS identification from positive blood cultures in routine diagnostics and effects on empiric antimicrobial therapy.Antimicrobial resistance and infection control · 2017Article
- A new rapid method for direct antimicrobial susceptibility testing of bacteria from positive blood cultures.BMC microbiology · 2016Article
- Enrichment of bacterial DNA for the diagnosis of blood stream infections.BMC infectious diseases · 2016Article
- [Severe pneumonia in the intensive care unit].Medizinische Klinik, Intensivmedizin und Notfallmedizin · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polymerase chain reaction (PCR)-based techniques allow more rapid and sensitive detection of pathogens compared with conventional blood culture. Nevertheless, the climate of opinion of relevant studies is that currently PCR can supplement but not replace blood culture. In numerous studies, combined detection rate of both methods was significantly higher compared with PCR or blood culture alone. Also, complete determination of antibiotic resistance can currently be performed only by blood culture. Further increase of the panel of multiplex PCR is complicated, because the vast majority of sepsis pathogens are already included, primer interactions leading to primer heteromers limit the amount of targets detectable within one PCR tube, and an array of too many individual PCR reactions for investigation of a single specimen leads to high cost and workload. Except for diagnostics of patients in whom unusual, not culturable, or fastidious pathogens are detected more often, such as immunosuppressed patients with suspected parasitic infection, etc., it might even not be necessary to further increase the spectrum of detectable species. If the primary aim of PCR diagnostics is to decrease inappropriate empirical treatment and improve patient outcome, detection should focus on those pathogens or resistance determinants that are not covered by guideline-recommended treatment regimens and that have been identified as the major cause of inappropriate treatment according to current studies. In our opinion, such a narrower assay is more cost effective, may achieve higher accuracy due to reduced intratest interference, and would better address current and emerging clinical needs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.