ArticleMolecules and cells2011
Differential expression of extracellular matrix proteins in senescent and young human fibroblasts: a comparative proteomics and microarray study.
Article in Molecules and cells, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Hallmarks of the ageing lung: 10 years later.The European respiratory journal · 2026Review
- Cancer-cell-secreted DDAH1 induces TGF-β1/Smad3 signaling pathway to promote fibrosis and aging in lung.Nature aging · 2026Article
- The aging lung: microenvironment, mechanisms, and diseases.Frontiers in immunology · 2024Review
- The aged extracellular matrix and the profibrotic role of senescence-associated secretory phenotype.American journal of physiology. Cell physiology · 2023Review
- Impact of aging on immune function in the pathogenesis of pulmonary diseases: potential for therapeutic targets.Expert review of respiratory medicine · 2023Review
- Review
- The Flower Extract ofJournal of microbiology and biotechnology · 2020Article
- Quantitative proteomic profiling of extracellular matrix and site-specific collagen post-translational modifications in anMatrix biology plus · 2019Article
- Article
- Molecular insights into the premature aging disease progeria.Histochemistry and cell biology · 2016Review
- Article
- Single cell analysis reveals the stochastic phase of reprogramming to pluripotency is an ordered probabilistic process.PloS one · 2014Article
- Insulin-like growth factor binding proteins 4 and 7 released by senescent cells promote premature senescence in mesenchymal stem cells.Cell death & disease · 2013Article
- Defining the extracellular matrix using proteomics.International journal of experimental pathology · 2013Review
- Senescent cells and their secretory phenotype as targets for cancer therapy.Interdisciplinary topics in gerontology · 2013Review
- Survey of the Applications of NGS to Whole-Genome Sequencing and Expression Profiling.Genomics & informatics · 2012Article
- Ageing, cellular senescence and the impact of diet: an overview.Porto biomedical journalReview
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
The extracellular matrix (ECM) provides an essential structural framework for cell attachment, proliferation, and differentiation, and undergoes progressive changes during senescence. To investigate changes in protein expression in the extracellular matrix between young and senescent fibroblasts, we compared proteomic data (LTQ-FT) with cDNA microarray results. The peptide counts from the proteomics analysis were used to evaluate the level of ECM protein expression by young cells and senescent cells, and ECM protein expression data were compared with the microarray data. After completing the comparative analysis, we grouped the genes into four categories. Class I included genes with increased expression levels in both analyses, while class IV contained genes with reduced expression in both analyses. Class II and Class III contained genes with an inconsistent expression pattern. Finally, we validated the comparative analysis results by examining the expression level of the specific gene from each category using Western blot analysis and semiquantitative RT-PCR. Our results demonstrate that comparative analysis can be used to identify differentially expressed genes.
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