ArticlePloS one2011
A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2,894 papers.
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Who cites it
2,894 citing papers in PubMed.
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- Chromosome-level genome of wild-simulated Panax ginseng identifies SNP markers for germplasm and medicinal quality evaluation.Journal of advanced research · 2026Article
- Clonality, management and geography shape genetic structure in a perennial species with restricted distribution.Annals of botany · 2026Article
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- Genomic selection in timothy (Phleum pratense L.): a comprehensive evaluation of prediction models, multi-trait strategies, and forward validation across Norwegian environments.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Contrasting Patterns of Genetic Isolation Among Diadromous, Landlocked, and Non-Migratory Gobiid Fish Populations.Molecular ecology · 2026Article
- Development of an open-pollinated genetic mapping framework to facilitate the identification of QTL in apples.The plant genome · 2026Article
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- Recessive antimorph alleles reveal novel functions of the Opaque1 myosin XI in maize.Plant physiology · 2026Article
- Fine-Scale Population Genomics Reveals Genetic Differentiation in the Brooding AmphipodEcology and evolution · 2026Article
- Standardized microhaplotype databases and frameworks for assessing and mining crop genetic diversity.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Genetic mapping identifies QTL and candidate genes including Mendel's A gene controlling high levels of partial resistance to Fusarium avenaceum in Pea.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Genomic differentiation and SNP variation reveal local adaptations to eastern Mediterranean environmental conditions in wild radishes.Annals of botany · 2026Article
2,834 more citing papers are in PubMed but not listed here.
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7 authors.
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Abstract
Advances in next generation technologies have driven the costs of DNA sequencing down to the point that genotyping-by-sequencing (GBS) is now feasible for high diversity, large genome species. Here, we report a procedure for constructing GBS libraries based on reducing genome complexity with restriction enzymes (REs). This approach is simple, quick, extremely specific, highly reproducible, and may reach important regions of the genome that are inaccessible to sequence capture approaches. By using methylation-sensitive REs, repetitive regions of genomes can be avoided and lower copy regions targeted with two to three fold higher efficiency. This tremendously simplifies computationally challenging alignment problems in species with high levels of genetic diversity. The GBS procedure is demonstrated with maize (IBM) and barley (Oregon Wolfe Barley) recombinant inbred populations where roughly 200,000 and 25,000 sequence tags were mapped, respectively. An advantage in species like barley that lack a complete genome sequence is that a reference map need only be developed around the restriction sites, and this can be done in the process of sample genotyping. In such cases, the consensus of the read clusters across the sequence tagged sites becomes the reference. Alternatively, for kinship analyses in the absence of a reference genome, the sequence tags can simply be treated as dominant markers. Future application of GBS to breeding, conservation, and global species and population surveys may allow plant breeders to conduct genomic selection on a novel germplasm or species without first having to develop any prior molecular tools, or conservation biologists to determine population structure without prior knowledge of the genome or diversity in the species.
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