ArticlePloS one2011
sodC-based real-time PCR for detection of Neisseria meningitidis.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers, 1 of them a synthesis that pooled it.
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Who cites it
62 citing papers in PubMed, 1 synthesis or guideline pooled it, 105 citations in OpenAlex.
- Serogroup-specific meningococcal carriage by age group: a systematic review and meta-analysis.BMJ open · 2019Pooled it
- Risk Factors and Prevalence of Meningococcal Colonisation of Children Attending Kindergarten in Northern Hungary.Microorganisms · 2026Article
- Genomic Typing of Meningococcal Carriage Isolates in an Urban Sexual Health Clinic.Pathogens (Basel, Switzerland) · 2026Article
- A novel real-time PCR assay for the simultaneous detection of the four main causes of bacterial meningitis.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2026Article
- Prevalence ofOpen forum infectious diseases · 2025Article
- Utility of superoxide dismutase C (Microbiology spectrum · 2025Article
- Performance Comparison of Two In-House PCR Methods for DetectingDiagnostics (Basel, Switzerland) · 2025Article
- Article
- Expansion of Neisseria meningitidis Serogroup C Clonal Complex 10217 during Meningitis Outbreak, Burkina Faso, 2019.Emerging infectious diseases · 2024Article
- Tracing the Negative Results of Multiplex Real-Time PCR Assay for Diagnosis of Bacterial Pediatrics Meningitis.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2023Article
- Enhancing Meningococcal Genomic Surveillance in the Meningitis Belt Using High-Resolution Culture-Free Whole-Genome Sequencing.The Journal of infectious diseases · 2022Article
- Invasive Meningococcal X Disease during the COVID-19 Pandemic, Brazil.Emerging infectious diseases · 2022Article
- Limitation ofJournal of clinical microbiology · 2022Article
- Evaluation of Urethrotropic-Clade Meningococcal Infection by Urine Metagenomic Shotgun Sequencing.Journal of clinical microbiology · 2022Article
- Occurrence of influenza and bacterial infections in cancer patients receiving radiotherapy in Ghana.PloS one · 2022Article
- Genomic Insights on Variation Underlying Capsule Expression in Meningococcal Carriage Isolates From University Students, United States, 2015-2016.Frontiers in microbiology · 2022Article
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- Human Immunodeficiency Virus Infection Is Associated With Increased Meningococcal Carriage Acquisition Among First-year Students in 2 South African Universities.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Article
- Molecular diagnostic assays for the detection of common bacterial meningitis pathogens: A narrative review.EBioMedicine · 2021Review
2 more citing papers are in PubMed but not listed here.
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Authors and funding
26 authors at 8 institutions in 4 countries.
Funding
Abstract
Real-time PCR (rt-PCR) is a widely used molecular method for detection of Neisseria meningitidis (Nm). Several rt-PCR assays for Nm target the capsule transport gene, ctrA. However, over 16% of meningococcal carriage isolates lack ctrA, rendering this target gene ineffective at identification of this sub-population of meningococcal isolates. The Cu-Zn superoxide dismutase gene, sodC, is found in Nm but not in other Neisseria species. To better identify Nm, regardless of capsule genotype or expression status, a sodC-based TaqMan rt-PCR assay was developed and validated. Standard curves revealed an average lower limit of detection of 73 genomes per reaction at cycle threshold (C(t)) value of 35, with 100% average reaction efficiency and an average R(2) of 0.9925. 99.7% (624/626) of Nm isolates tested were sodC-positive, with a range of average C(t) values from 13.0 to 29.5. The mean sodC C(t) value of these Nm isolates was 17.6±2.2 (±SD). Of the 626 Nm tested, 178 were nongroupable (NG) ctrA-negative Nm isolates, and 98.9% (176/178) of these were detected by sodC rt-PCR. The assay was 100% specific, with all 244 non-Nm isolates testing negative. Of 157 clinical specimens tested, sodC detected 25/157 Nm or 4 additional specimens compared to ctrA and 24 more than culture. Among 582 carriage specimens, sodC detected Nm in 1 more than ctrA and in 4 more than culture. This sodC rt-PCR assay is a highly sensitive and specific method for detection of Nm, especially in carriage studies where many meningococcal isolates lack capsule genes.
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