ArticlePLoS pathogens2011
Viral small interfering RNAs target host genes to mediate disease symptoms in plants.
Article in PLoS pathogens, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
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Who cites it
113 citing papers in PubMed, 258 citations in OpenAlex.
- Genome-Wide Screening and Functional Verification of the Tobacco SGR Family Identify Transcription-Factor-Like NtSGRL as a Negative Regulator of Tomato Yellow Leaf Curl Virus Resistance.Molecular plant pathology · 2026Article
- Satellite RNAs of Cucumber Mosaic Virus: Molecular Features, Pathogenic Roles, and Ecological Dynamics.Current microbiology · 2026Review
- Small RNA mobility and plant virus diseases.Journal of experimental botany · 2026Review
- Small RNA-mediated regulation of cross-kingdom gene expression in sugar beet genotypes resistant and susceptible to rhizomania.The Journal of general virology · 2025Article
- Cucumber Green Mottle Mosaic Virus Decreases ChlorophyllPlants (Basel, Switzerland) · 2025Article
- Decoding the Dialog Between Plants and Arbuscular Mycorrhizal Fungi: A Molecular Genetic Perspective.Genes · 2025Review
- The crucial role of mitochondrial/chloroplast-related genes in viral genome replication and host defense: integrative systems biology analysis in plant-virus interaction.Frontiers in microbiology · 2025Article
- A phytoplasma effector destabilizes chloroplastic glutamine synthetase inducing chlorotic leaves that attract leafhopper vectors.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The plant siRNA landscape.The Plant cell · 2024Review
- Aphid-mediated beet yellows virus transmission initiates proviral gene deregulation in sugar beet at early stages of infection.PloS one · 2024Article
- Profiling of small RNAs derived from tomato brown rugose fruit virus in infectedFrontiers in microbiology · 2024Article
- Beyond Loading: Functions of Plant ARGONAUTE Proteins.International journal of molecular sciences · 2023Review
- VIGS as a strategy to reverse aphid wing induction by Y-satellite RNA of cucumber mosaic virus.FEBS open bio · 2023Article
- Identification of Host Factors Interacting with a γ-Shaped RNA Element from a Plant Virus-Associated Satellite RNA.Viruses · 2023Article
- Unraveling the Mechanisms of Virus-Induced Symptom Development in Plants.Plants (Basel, Switzerland) · 2023Review
- Small Talk: On the Possible Role of Trans-Kingdom Small RNAs during Plant-Virus-Vector Tritrophic Communication.Plants (Basel, Switzerland) · 2023Review
- Maize Lethal Necrosis disease: review of molecular and genetic resistance mechanisms, socio-economic impacts, and mitigation strategies in sub-Saharan Africa.BMC plant biology · 2022Review
- Flexible and digestible wood caused by viral-induced alteration of cell wall composition.Current biology : CB · 2022Article
- Application progress of plant-mediated RNAi in pest control.Frontiers in bioengineering and biotechnology · 2022Review
- Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses.Methods in molecular biology (Clifton, N.J.) · 2022Article
53 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cucumber mosaic virus (CMV) Y-satellite RNA (Y-Sat) has a small non-protein-coding RNA genome that induces yellowing symptoms in infected Nicotiana tabacum (tobacco). How this RNA pathogen induces such symptoms has been a longstanding question. We show that the yellowing symptoms are a result of small interfering RNA (siRNA)-directed RNA silencing of the chlorophyll biosynthetic gene, CHLI. The CHLI mRNA contains a 22-nucleotide (nt) complementary sequence to the Y-Sat genome, and in Y-Sat-infected plants, CHLI expression is dramatically down-regulated. Small RNA sequencing and 5' RACE analyses confirmed that this 22-nt sequence was targeted for mRNA cleavage by Y-Sat-derived siRNAs. Transformation of tobacco with a RNA interference (RNAi) vector targeting CHLI induced Y-Sat-like symptoms. In addition, the symptoms of Y-Sat infection can be completely prevented by transforming tobacco with a silencing-resistant variant of the CHLI gene. These results suggest that siRNA-directed silencing of CHLI is solely responsible for the Y-Sat-induced symptoms. Furthermore, we demonstrate that two Nicotiana species, which do not develop yellowing symptoms upon Y-Sat infection, contain a single nucleotide polymorphism within the siRNA-targeted CHLI sequence. This suggests that the previously observed species specificity of Y-Sat-induced symptoms is due to natural sequence variation in the CHLI gene, preventing CHLI silencing in species with a mismatch to the Y-Sat siRNA. Taken together, these findings provide the first demonstration of small RNA-mediated viral disease symptom production and offer an explanation of the species specificity of the viral disease.
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