ArticlePloS one2011
Characterizing mRNA interactions with RNA granules during translation initiation inhibition.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 50 citations in OpenAlex.
- Improved detection and consistency of RNA-interacting proteomes using DIA SILAC.Nucleic acids research · 2024Article
- Avidity-based bright and photostable light-up aptamers for single-molecule mRNA imaging.Nature chemical biology · 2023Article
- Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM).Nature communications · 2023Article
- Aging RNA granule dynamics in neurodegeneration.Frontiers in molecular biosciences · 2022Review
- Coupling of translation quality control and mRNA targeting to stress granules.The Journal of cell biology · 2020Article
- A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells.Methods in enzymology · 2020Article
- Drug-induced Stress Granule Formation Protects Sensory Hair Cells in Mouse Cochlear Explants During Ototoxicity.Scientific reports · 2019Article
- Principles of Stress Granules Revealed by Imaging Approaches.Cold Spring Harbor perspectives in biology · 2019Review
- Nuclear Tau, p53 and Pin1 Regulate PARN-Mediated Deadenylation and Gene Expression.Frontiers in molecular neuroscience · 2019Article
- The Role of RNA in Biological Phase Separations.Journal of molecular biology · 2018Review
- A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells.Nature chemical biology · 2018Article
- Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule AssemblyFrontiers in immunology · 2018Article
- Characterizing exogenous mRNA delivery, trafficking, cytoplasmic release and RNA-protein correlations at the level of single cells.Nucleic acids research · 2017Article
- Attacked from All Sides: RNA Decay in Antiviral Defense.Viruses · 2017Review
- A Novel Method to Quantify RNA-Protein Interactions In Situ Using FMTRIP and Proximity Ligation.Methods in molecular biology (Clifton, N.J.) · 2017Article
- CD-tagging-MS2: detecting allelic expression of endogenous mRNAs and their protein products in single cells.Biology methods & protocols · 2017Article
- Deletion of afpab1 Causes Increased Sensitivity to Oxidative Stress and Hypovirulence in Aspergillus fumigatus.International journal of molecular sciences · 2016Article
- Review
- Can we observe changes in mRNA "state"? Overview of methods to study mRNA interactions with regulatory proteins relevant in cancer related processes.The Analyst · 2016Review
- The BEACH Domain Protein SPIRRIG Is Essential for Arabidopsis Salt Stress Tolerance and Functions as a Regulator of Transcript Stabilization and Localization.PLoS biology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
When cells experience environmental stresses, global translational arrest is often accompanied by the formation of stress granules (SG) and an increase in the number of p-bodies (PBs), which are thought to play a crucial role in the regulation of eukaryotic gene expression through the control of mRNA translation and degradation. SGs and PBs have been extensively studied from the perspective of their protein content and dynamics but, to date, there have not been systematic studies on how they interact with native mRNA granules. Here, we demonstrate the use of live-cell hybridization assays with multiply-labeled tetravalent RNA imaging probes (MTRIPs) combined with immunofluorescence, as a tool to characterize the polyA+ and β-actin mRNA distributions within the cytoplasm of epithelial cell lines, and the changes in their colocalization with native RNA granules including SGs, PBs and the RNA exosome during the inhibition of translational initiation. Translation initiation inhibition was achieved via the induction of oxidative stress using sodium arsenite, as well as through the use of Pateamine A, puromycin and cycloheximide. This methodology represents a valuable tool for future studies of mRNA trafficking and regulation within living cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.