ArticlePloS one2011
Two distinct integrin-mediated mechanisms contribute to apical lumen formation in epithelial cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 59 citations in OpenAlex.
- Multiscale imaging of polarity in bovine oviductal organoids.Frontiers in cell and developmental biology · 2026Article
- FAK mediates mechanical signaling to maintain epithelial homeostasis through YAP/TAZ-TEADs.Histochemistry and cell biology · 2025Article
- The scaffold protein IQGAP1 promotes reorientation of epithelial cell polarity at the two-cell stage for cystogenesis.Genes to cells : devoted to molecular & cellular mechanisms · 2024Article
- HIF2α-dependent Dock4/Rac1-signaling regulates formation of adherens junctions and cell polarity in normoxia.Scientific reports · 2024Article
- A computational analysis of the role of integrins and Rho-GTPases in the emergence and disruption of apical-basal polarization in renal epithelial cells.PLoS computational biology · 2024Article
- Persistent cell contacts enable E-cadherin/HMR-1- and PAR-3-based symmetry breaking within a developing C. elegans epithelium.Developmental cell · 2023Article
- A DLG1-ARHGAP31-CDC42 axis is essential for the intestinal stem cell response to fluctuating niche Wnt signaling.Cell stem cell · 2023Article
- Synthetic Collagen-like Polymer That Undergoes a Sol-Gel Transition Triggered byInternational journal of molecular sciences · 2022Article
- Substrate Stiffness Determines the Establishment of Apical-Basal Polarization in Renal Epithelial Cells but Not in Tubuloid-Derived Cells.Frontiers in bioengineering and biotechnology · 2022Article
- Dissection, Fixation, and Immunostaining of the Drosophila Midgut.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Towards organoid culture without Matrigel.Communications biology · 2021Review
- Tunable Hybrid Matrices Drive Epithelial Morphogenesis and YAP Translocation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Adhesion of Epithelial Cells to PNIPAm Treated Surfaces for Temperature-Controlled Cell-Sheet Harvesting.ACS applied materials & interfaces · 2020Article
- Article
- Connexin 43 maintains tissue polarity and regulates mitotic spindle orientation in the breast epithelium.Journal of cell science · 2019Article
- Epithelial polarization in 3D matrix requires DDR1 signaling to regulate actomyosin contractility.Life science alliance · 2019Article
- Assembly of the β4-Integrin Interactome Based on Proximal Biotinylation in the Presence and Absence of Heterodimerization.Molecular & cellular proteomics : MCP · 2019Article
- Mechanochemical Signaling of the Extracellular Matrix in Epithelial-Mesenchymal Transition.Frontiers in cell and developmental biology · 2019Review
- An alternative mode of epithelial polarity in the Drosophila midgut.PLoS biology · 2018Article
- Towards a defined ECM and small molecule based monolayer culture system for the expansion of mouse and human intestinal stem cells.Biomaterials · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFormation of apical compartments underlies the morphogenesis of most epithelial organs during development. The extracellular matrix (ECM), particularly the basement membrane (BM), plays an important role in orienting the apico-basal polarity and thereby the positioning of apical lumens. Integrins have been recognized as essential mediators of matrix-derived polarity signals. The importance of β1-integrins in epithelial polarization is well established but the significance of the accompanying α-subunits have not been analyzed in detail. PRINCIPAL
findingsHere we demonstrate that two distinct integrin-dependent pathways regulate formation of apical lumens to ensure robust apical membrane biogenesis under different microenvironmental conditions; 1) α2β1- and α6β4-integrins were required to establish a basal cue that depends on Rac1-activity and guides apico-basal cell polarization. 2) α3β1-integrins were implicated in positioning of mitotic spindles in cysts, a process that is essential for Cdc42-driven epithelial hollowing. SIGNIFICANCE: Identification of the separate processes driven by particular integrin receptors clarifies the functional hierarchies between the different integrins co-expressed in epithelial cells and provides valuable insight into the complexity of cell-ECM interactions thereby guiding future studies addressing the molecular basis of epithelial morphogenesis during development and disease.
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