ArticlePloS one2011
Sequencing-based approaches reveal low ambient temperature-responsive and tissue-specific microRNAs in phalaenopsis orchid.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 77 citations in OpenAlex.
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- Genome-Wide Identification and Expression Analysis of the SPL Gene Family in Three Orchids.International journal of molecular sciences · 2023Article
- The Integrated mRNA and miRNA Approach Reveals Potential Regulators of Flowering Time inInternational journal of molecular sciences · 2023Article
- Transcriptome Analysis Reveals Endogenous Hormone Changes during Spike Development inInternational journal of molecular sciences · 2022Article
- Flowering time runs hot and cold.Plant physiology · 2022Review
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- Advances and prospects of orchid research and industrialization.Horticulture research · 2022Article
- Genome-wide miRNA analysis and integrated network for flavonoid biosynthesis in Osmanthus fragrans.BMC genomics · 2021Article
- Small RNA and Transcriptome Sequencing Reveals miRNA Regulation of Floral Thermogenesis inInternational journal of molecular sciences · 2020Article
- Molecular Characterization and Expression Profile ofPlants (Basel, Switzerland) · 2020Article
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- Small RNAs, emerging regulators critical for the development of horticultural traits.Horticulture research · 2018Article
- Post genomics era for orchid research.Botanical studies · 2017Review
- Integrated mRNA and microRNA transcriptome variations in the multi-tepal mutant provide insights into the floral patterning of the orchid Cymbidium goeringii.BMC genomics · 2017Article
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- Parsing the Regulatory Network between Small RNAs and Target Genes in Ethylene Pathway in Tomato.Frontiers in plant science · 2017Article
- Small RNA transcriptomes of mangroves evolve adaptively in extreme environments.Scientific reports · 2016Article
- Article
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- Genetic Architecture of Natural Variation in Thermal Responses of Arabidopsis.Plant physiology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant small RNAs (smRNAs) are short, non-coding RNA molecules that mediate RNA silencing and regulate a group of genes involved in plant development and responses to environmental stimuli. Low temperature is necessary to initiate stalk development in the orchid Phalaenopsis aphrodite subsp. formosana. To identify smRNAs in Phalaenopsis responding to low temperatures, a smRNA profiling analysis using high-throughput sequencing technology was performed. Subsequent bioinformatic analysis was applied to categorize the miRNAs identified. A total of 37,533,509 smRNA reads yielded 11,129 independent orchid miRNA sequences, representing 329 known miRNA families identified in other plant species. Because the genomic resources available for Phalaenopsis are limited, a transcriptomic database was established using deep sequencing data sets to identify miRNAs precursors and their target transcripts. Comparing small RNAs and the transcriptomic database, 14 putative miRNA precursors of 10 miRNA families were identified, as were hundreds of putative targets. Comparing sequencing data and smRNA northern hybridization results identified miR156, miR162, miR528 and miR535 as low temperature-induced miRNAs. In addition, tissue-specific expression of these miRNAs was investigated. It was concluded that miR156 and miR172 may be components of a regulatory pathway mediating transition from the vegetative to the reproductive phase in Phalaenopsis. The smRNA and transcriptomic databases could be the foundations for further research aimed at elucidating the control of the flowering time in orchids.
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