ArticlePloS one2011
Fur activates the expression of Salmonella enterica pathogenicity island 1 by directly interacting with the hilD operator in vivo and in vitro.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed.
- Study in the iron uptake mechanism of Pasteurella multocida.Veterinary research · 2025Review
- Article
- FoxR is an AraC-like transcriptional regulator of ferrioxamine uptake in Salmonella enterica.Molecular microbiology · 2022Article
- Interplay between Rho, H-NS, spurious transcription, andProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Structural basis of Streptomyces transcription activation by zinc uptake regulator.Nucleic acids research · 2022Article
- Article
- Fur functions as an activator of T6SS-mediated bacterial dominance and virulence inFrontiers in microbiology · 2022Article
- Iron Transport and Metabolism in Escherichia, Shigella, and Salmonella.EcoSal Plus · 2021Article
- The FUR-like regulators PerRA and PerRB integrate a complex regulatory network that promotes mammalian host-adaptation and virulence of Leptospira interrogans.PLoS pathogens · 2021Article
- Long-Distance Effects of H-NS Binding in the Control ofJournal of bacteriology · 2021Article
- The battle for iron in enteric infections.Immunology · 2020Review
- Bacillus subtilis Fur Is a Transcriptional Activator for the PerR-RepressedJournal of bacteriology · 2020Article
- Functional Insights From KpfR, a New Transcriptional Regulator of Fimbrial Expression That Is Crucial forFrontiers in microbiology · 2020Article
- The Ferric Uptake Regulator Represses Type VI Secretion System Function by Binding Directly to theInfection and immunity · 2019Article
- PhoP-Mediated Repression of the SPI1 Type 3 Secretion System in Salmonella enterica Serovar Typhimurium.Journal of bacteriology · 2019Article
- Oxygen-dependent regulation of SPI1 type three secretion system by small RNAs in Salmonella enterica serovar Typhimurium.Molecular microbiology · 2019Article
- Identification of Fur inFrontiers in veterinary science · 2019Article
- Review
- Zinc-dependent regulation of zinc import and export genes by Zur.Nature communications · 2017Article
- Global Gene-expression Analysis of the Response ofFrontiers in microbiology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies have established that the expression of Salmonella enterica pathogenicity island 1 (SPI1), which is essential for epithelial invasion, is mainly regulated by the HilD protein. The ferric uptake regulator, Fur, in turn modulates the expression of the S. enterica hilD gene, albeit through an unknown mechanism. Here we report that S. enterica Fur, in its metal-bound form, specifically binds to an AT-rich region (BoxA), located upstream of the hilD promoter (P(hilD)), at position -191 to -163 relative to the hilD transcription start site. Furthermore, in a P(hilD) variant with mutations in BoxA, P(hilD*), Fur·Mn(2+) binding is impaired. In vivo experiments using S. enterica strains carrying wild-type P(hilD) or the mutant variant P(hilD*) showed that Fur activates hilD expression, while in vitro experiments revealed that the Fur·Mn(2+) protein is sufficient to increase hilD transcription. Together, these results present the first evidence that Fur·Mn(2+), by binding to the upstream BoxA sequence, directly stimulates the expression of hilD in S. enterica.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.