ArticlePloS one2011
A non-specific effect associated with conditional transgene expression based on Cre-loxP strategy in mice.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types.Cell · 2025Article
- A Study on Potential Sources of Perineuronal Net-Associated Sema3A in Cerebellar Nuclei Reveals Toxicity of Non-Invasive AAV-Mediated Cre Expression in the Central Nervous System.International journal of molecular sciences · 2025Article
- A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types.bioRxiv : the preprint server for biology · 2024Article
- Aberrant glial activation and synaptic defects in CaMKIIα-iCre and nestin-Cre transgenic mouse models.Scientific reports · 2022Article
- Developmental impact of glutamate transporter overexpression on dopaminergic neuron activity and stereotypic behavior.Molecular psychiatry · 2022Article
- Article
- Podocyte-specific expression of Cre recombinase promotes glomerular basement membrane thickening.American journal of physiology. Renal physiology · 2019Article
- Mitochondrial Complex I Function Is Essential for Neural Stem/Progenitor Cells Proliferation and Differentiation.Frontiers in neuroscience · 2019Article
- Creating conditional dual fluorescence labeled transgenic animals for studying function of small noncoding RNAs.Connective tissue research · 2017Article
- Expression and functional analysis of the Wnt/beta-catenin induced mir-135a-2 locus in embryonic forebrain development.Neural development · 2016Article
- Metabolic and Behavioural Phenotypes in Nestin-Cre Mice Are Caused by Hypothalamic Expression of Human Growth Hormone.PloS one · 2015Article
- Cellular FLICE-like inhibitory protein protects against cardiac hypertrophy by blocking ASK1/p38 signaling in mice.Molecular and cellular biochemistry · 2014Article
- Partial loss of TDP-43 function causes phenotypes of amyotrophic lateral sclerosis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.Molecular neurodegeneration · 2013Article
- Selective overexpression of human SIRT1 in adipose tissue enhances energy homeostasis and prevents the deterioration of insulin sensitivity with ageing in mice.American journal of translational research · 2013Article
- Nitric oxide-mediated oxidative damage and the progressive demise of motor neurons in ALS.Neurotoxicity research · 2012Review
- Cardiac-specific inducible and conditional gene targeting in mice.Circulation research · 2012Review
- Construction of permanently inducible miRNA-based expression vectors using site-specific recombinases.BMC biotechnology · 2011Article
- Gene Editing in Human Pluripotent Stem Cells: Choosing the Correct Path.Journal of stem cell and regenerative biologyArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Transgenes flanked by loxP sites have been widely used to generate transgenic mice where the transgene expression can be controlled spatially and temporally by Cre recombinase. Data from this approach has led to important conclusions in cancer, neurodevelopment and neurodegeneration. Using this approach to conditionally express micro RNAs (miRNAs) in mice, we found that Cre-mediated recombination in neural progenitor cells caused microcephaly in five of our ten independent transgenic lines. This effect was not associated with the types or the quantity of miRNAs being expressed, nor was it associated with specific target knockdown. Rather, it was correlated with the presence of multiple tandem transgene copies and inverted (head-to-head or tail-to-tail) transgene repeats. The presence of these inverted repeats caused a high level of cell death in the ventricular zone of the embryonic brain, where Cre was expressed. Therefore, results from this Cre-loxP approach to generate inducible transgenic alleles must be interpreted with caution and conclusions drawn in previous reports may need reexamination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.