ArticleModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc2011
A comparison of clinically utilized human papillomavirus detection methods in head and neck cancer.
Article in Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers, 3 of them syntheses that pooled it.
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Who cites it
77 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Pooled it
- Meta-analysis of survival in patients with HNSCC discriminates risk depending on combined HPV and p16 status.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2016Pooled it
- Human papillomavirus in non-oropharyngeal head and neck cancers: a systematic literature review.Head and neck pathology · 2012Pooled it
- Identification of a 32-gene signature that determines HPV status in head and neck cancer.Discover oncology · 2026Article
- The role of human papillomavirus 16 in oral cavity and laryngeal cancers in the United States.Journal of the National Cancer Institute · 2025Article
- Diagnostic accuracy of HPV16 early antigen serology for HPV-driven oropharyngeal cancer is independent of age and sex.International journal of cancer · 2024Article
- Clinicopathologic characterization of cervical metastasis from an unknown primary tumor: a multicenter study in Korea.Journal of pathology and translational medicine · 2023Article
- Single cell transcriptomic analysis of HPV16-infected epithelium identifies a keratinocyte subpopulation implicated in cancer.Nature communications · 2023Article
- A Case of HPV-Associated Oropharyngeal Squamous Cell Carcinoma with Block-Like, Partial Loss of p16 Expression.Head and neck pathology · 2022Article
- Internal heating method of loop-mediated isothermal amplification for detection of HPV-6 DNA.Mikrochimica acta · 2022Article
- Influence of tumor mutational burden, inflammatory gene expression profile, and PD-L1 expression on response to pembrolizumab in head and neck squamous cell carcinoma.Journal for immunotherapy of cancer · 2022Article
- Head and Neck Squamous Cell Carcinoma: NT5E Could Be a Prognostic Biomarker.Applied bionics and biomechanics · 2022Article
- Localization and genotyping of canine papillomavirus in canine inverted papillomas.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- A New Look into Cancer-A Review on the Contribution of Vibrational Spectroscopy on Early Diagnosis and Surgery Guidance.Cancers · 2021Review
- Genomic Characterization and Therapeutic Targeting of HPV Undetected Cervical Carcinomas.Cancers · 2021Article
- Human papillomavirus-16 infection and p16 expression in oral squamous cell carcinoma.Oncology letters · 2021Article
- CD68Frontiers in oncology · 2021Article
- Immune checkpoint status and tumor microenvironment in vulvar squamous cell carcinoma.Virchows Archiv : an international journal of pathology · 2020Article
- Importance of investigating high-risk human papillomavirus in lymph node metastasis of esophageal adenocarcinoma.World journal of gastroenterology · 2020Review
- Comparative Performance of High-Risk Human Papillomavirus RNA and DNA In Situ Hybridization on College of American Pathologists Proficiency Tests.Archives of pathology & laboratory medicine · 2020Article
17 more citing papers are in PubMed but not listed here.
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9 authors.
Funding
Abstract
Detection of human papillomavirus (HPV) in head and neck cancer has therapeutic implications. In situ hybridization and immunohistochemistry for p16 are used by surgical pathologists. We compared the sensitivity and specificity of three popular commercial tests for HPV detection in head and neck squamous cell carcinomas with a 'gold standard' HPV PCR assay. A total of 110 prospectively collected, formalin-fixed tumor specimens were compiled onto tissue microarrays and tested for HPV DNA by in situ hybridization with two probe sets, a biotinylated probe for high-risk (HR) HPV types 16/18 (Dako, CA, USA) and a probe cocktail for 16/18, plus 10 additional HR types (Ventana, AZ, USA). The p16(INK4) expression was also assessed using a Pharmingen immunohistochemistry antibody (BD Biosciences, CA, USA). Tissue microarrays were stained and scored at expert laboratories. HPV DNA was detected by MY09/11-PCR, using Gold AmpliTaq and dot-blot hybridization on matched-fresh frozen specimens in a research laboratory. HPV 16 E6 and E7-RNA expression was also measured using RT-PCR. Test performance was assessed by a receiver operating characteristic analysis. HR-HPV DNA types 16, 18 and 35 were detected by MY-PCR in 28% of tumors, with the majority (97%) testing positive for type 16. Compared with MY-PCR, the sensitivity and specificity for HR-HPV DNA detection with Dako in situ hybridization was 21% (95% confidence interval (CI): 7-42) and 100% (95% CI: 93-100), respectively. Corresponding test results by Ventana in situ hybridization were 59% (95% CI: 39-78) and 58% (95% CI: 45-71), respectively. The p16 immunohistochemistry performed better overall than Dako (P=0.042) and Ventana (P=0.055), with a sensitivity of 52% (95% CI: 32-71) and specificity of 93% (95% CI: 84-98). Compared with a gold standard HPV-PCR assay, HPV detection by in situ hybridization was less accurate for head and neck squamous cell carcinoma on tissue microarrays than p16 immunohistochemistry. Further testing is warranted before these assays should be recommended for clinical HPV detection.
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