ArticleThe Journal of biological chemistry2011
CmRBP50 protein phosphorylation is essential for assembly of a stable phloem-mobile high-affinity ribonucleoprotein complex.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
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- Tandem Expression of a Mobile RNA and Its RNA-Binding Protein(s) Enhances Tuber Productivity in Potato.International journal of molecular sciences · 2023Article
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- The Landscape of RNA-Protein Interactions in Plants: Approaches and Current Status.International journal of molecular sciences · 2021Review
- RNA Motifs and Modification Involve in RNA Long-Distance Transport in Plants.Frontiers in cell and developmental biology · 2021Review
- Mobility of Antiflorigen and PEBP mRNAs in Tomato-Tobacco Heterografts.Plant physiology · 2018Article
- Selective Targeting of Mobile mRNAs to Plasmodesmata for Cell-to-Cell Movement.Plant physiology · 2018Article
- Nitrate-Regulated Glutaredoxins Control Arabidopsis Primary Root Growth.Plant physiology · 2016Article
- Phloem RNA-binding proteins as potential components of the long-distance RNA transport system.Frontiers in plant science · 2013Article
- Promoter activity of polypyrimidine tract-binding protein genes of potato responds to environmental cues.Planta · 2012Article
- Polypyrimidine tract binding protein homologs from Arabidopsis are key regulators of alternative splicing with implications in fundamental developmental processes.The Plant cell · 2012Article
- Overexpression of Arabidopsis plasmodesmata germin-like proteins disrupts root growth and development.The Plant cell · 2012Article
- The Role of Polypyrimidine Tract-Binding Proteins and Other hnRNP Proteins in Plant Splicing Regulation.Frontiers in plant science · 2012Article
- Mobile FT mRNA contributes to the systemic florigen signalling in floral induction.Scientific reports · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-binding proteins (RBPs) form ribonucleoprotein (RNP) complexes that play crucial roles in RNA processing for gene regulation. The angiosperm sieve tube system contains a unique population of transcripts, some of which function as long-distance signaling agents involved in regulating organ development. These phloem-mobile mRNAs are translocated as RNP complexes. One such complex is based on a phloem RBP named Cucurbita maxima RNA-binding protein 50 (CmRBP50), a member of the polypyrimidine track binding protein family. The core of this RNP complex contains six additional phloem proteins. Here, requirements for assembly of this CmRBP50 RNP complex are reported. Phosphorylation sites on CmRBP50 were mapped, and then coimmunoprecipitation and protein overlay studies established that the phosphoserine residues, located at the C terminus of CmRBP50, are critical for RNP complex assembly. In vitro pull-down experiments revealed that three phloem proteins, C. maxima phloem protein 16, C. maxima GTP-binding protein, and C. maxima phosphoinositide-specific phospholipase-like protein, bind directly with CmRBP50. This interaction required CmRBP50 phosphorylation. Gel mobility-shift assays demonstrated that assembly of the CmRBP50-based protein complex results in a system having enhanced binding affinity for phloem-mobile mRNAs carrying polypyrimidine track binding motifs. This property would be essential for effective long-distance translocation of bound mRNA to the target tissues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.