ArticleThe Journal of biological chemistry2011
Characterization of the endonuclease and ATP-dependent flap endo/exonuclease of Dna2.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- Biochemical Mechanisms of Genetic Recombination and DNA Repair.Annual review of biochemistry · 2025Review
- The emerging determinants of replication fork stability.Nucleic acids research · 2021Review
- Article
- Ubiquitinated-PCNA protects replication forks from DNA2-mediated degradation by regulating Okazaki fragment maturation and chromatin assembly.Nature communications · 2020Article
- Multiple roles of DNA2 nuclease/helicase in DNA metabolism, genome stability and human diseases.Nucleic acids research · 2020Review
- Review
- Dna2 processes behind the fork long ssDNA flaps generated by Pif1 and replication-dependent strand displacement.Nature communications · 2018Article
- A DNA nick at Ku-blocked double-strand break ends serves as an entry site for exonuclease 1 (Exo1) or Sgs1-Dna2 in long-range DNA end resection.The Journal of biological chemistry · 2018Article
- Genetic Separation of Sae2 Nuclease Activity from Mre11 Nuclease Functions in Budding Yeast.Molecular and cellular biology · 2017Article
- Structural insights into the function of ZRANB3 in replication stress response.Nature communications · 2017Article
- The motor activity of DNA2 functions as an ssDNA translocase to promote DNA end resection.Genes & development · 2017Article
- Replication stress by Py-Im polyamides induces a non-canonical ATR-dependent checkpoint response.Nucleic acids research · 2014Article
- Identification of a novel human mitochondrial endo-/exonuclease Ddk1/c20orf72 necessary for maintenance of proper 7S DNA levels.Nucleic acids research · 2013Article
- The SOSS1 single-stranded DNA binding complex promotes DNA end resection in concert with Exo1.The EMBO journal · 2013Article
- The role of DNA exonucleases in protecting genome stability and their impact on ageing.Age (Dordrecht, Netherlands) · 2012Review
- Cross talk between the nuclease and helicase activities of Dna2: role of an essential iron-sulfur cluster domain.Nucleic acids research · 2012Article
- ZRANB3 is a structure-specific ATP-dependent endonuclease involved in replication stress response.Genes & development · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Two processes, DNA replication and DNA damage repair, are key to maintaining genomic fidelity. The Dna2 enzyme lies at the heart of both of these processes, acting in conjunction with flap endonuclease 1 and replication protein A in DNA lagging strand replication and with BLM/Sgs1 and MRN/X in double strand break repair. In vitro, Dna2 helicase and flap endo/exonuclease activities require an unblocked 5' single-stranded DNA end to unwind or cleave DNA. In this study we characterize a Dna2 nuclease activity that does not require, and in fact can create, 5' single-stranded DNA ends. Both endonuclease and flap endo/exonuclease are abolished by the Dna2-K677R mutation, implicating the same active site in catalysis. In addition, we define a novel ATP-dependent flap endo/exonuclease activity, which is observed only in the presence of Mn(2+). The endonuclease is blocked by ATP and is thus experimentally distinguishable from the flap endo/exonuclease function. Thus, Dna2 activities resemble those of RecB and AddAB nucleases even more closely than previously appreciated. This work has important implications for understanding the mechanism of action of Dna2 in multiprotein complexes, where dissection of enzymatic activities and cofactor requirements of individual components contributing to orderly and precise execution of multistep replication/repair processes depends on detailed characterization of each individual activity.
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