ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2011
Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Epidemiological and molecular characteristics of human parainfluenza virus in southern China during 2016-2020.Virologica Sinica · 2025Article
- Combined multiplex panel test results are a poor estimate of disease prevalence without adjustment for test error.PLoS computational biology · 2024Article
- Epidemiology of community-acquired pneumonia among hospitalised children in Indonesia: a multicentre, prospective study.BMJ open · 2022Article
- Co-infection of SARS-CoV-2 with other viral respiratory pathogens in Yogyakarta, Indonesia: A cross-sectional study.Annals of medicine and surgery (2012) · 2022Article
- Genetic analysis of human parainfluenza virus type 4 associated with severe acute respiratory infection in children in Luohe City, Henan Province, China, during 2017-2018.Archives of virology · 2021Article
- Viral burden and diversity in acute respiratory tract infections in hospitalized children in wet and dry zones of Sri Lanka.PloS one · 2021Article
- Respiratory viruses in individuals with a high frequency of animal exposure in southern and highland Vietnam.Journal of medical virology · 2020Article
- SARS-CoV-2 and co-infections detection in nasopharyngeal throat swabs of COVID-19 patients by metagenomics.The Journal of infection · 2020Article
- Epidemiology and Phylogenetic Analysis of Viral Respiratory Infections in Vietnam.Frontiers in microbiology · 2020Article
- Article
- DAMIAN: an open source bioinformatics tool for fast, systematic and cohort based analysis of microorganisms in diagnostic samples.Scientific reports · 2019Article
- Bacterial co-infection of the respiratory tract in ventilated children with bronchiolitis; a retrospective cohort study.BMC infectious diseases · 2019Article
- Article
- Viruses in Vietnamese Patients Presenting with Community-Acquired Sepsis of Unknown Cause.Journal of clinical microbiology · 2019Article
- Detection of six common human paramyxoviruses in patients with acute febrile respiratory symptoms using a novel multiplex real-time RT-PCR assay.Journal of medical virology · 2019Article
- Simultaneous detection of 15 respiratory pathogens with a fluorescence probe melting curve analysis-based multiplex real-time PCR assay.International journal of molecular epidemiology and genetics · 2019Article
- Treatment with broadly neutralizing influenza antibodies reduces severity of secondary pneumococcal pneumonia in mice.Journal of medical virology · 2018Article
- Rapid detection and monitoring of human coronavirus infections.New microbes and new infections · 2018Article
- Clinical and Molecular Epidemiology of Human Parainfluenza Viruses 1-4 in Children from Viet Nam.Scientific reports · 2018Article
- A Multiplex Asymmetric Reverse Transcription-PCR Assay Combined With an Electrochemical DNA Sensor for Simultaneously Detecting and Subtyping Influenza A Viruses.Frontiers in microbiology · 2018Article
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMultiplex real time PCR is increasingly used to diagnose respiratory viruses and has shown to be superior to traditional methods, like culture and antigen detection. However, comprehensive data on sensitivity, specificity and performance of the multiplex PCR compared to the single target PCR's is limited for most published respiratory multiplex real time PCR assays.
objectivesDevelopment and extensive analysis of an internally controlled multiplex real time rt-PCR for detection of respiratory viruses. STUDY
designThe assay was validated in comparison to single-target PCR's using plasmid targets and prospectively collected nasopharyngeal aspirates.
resultsUsing plasmid targets the multiplex format was found to be as least as sensitive and specific as the single-target PCR and no competition was observed when different targets were present at different amounts in one tube. Clinical validation showed high concordance for all viruses tested except for samples with low levels of enterovirus.
conclusionThis multiplex showed excellent specificities for all 14 respiratory viruses and sensitivity was high except for clinical samples with low levels of enterovirus.
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