ArticleJournal of molecular biology2011
Role of φ29 connector channel loops in late-stage DNA packaging.
Article in Journal of molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- In situ structures of the portal-neck-tail complex of bacteriophage T4 inform a viral genome positioning mechanism.Nature communications · 2026Article
- Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail.Science advances · 2025Article
- DNA/RNA hybrids avoid channel gating that leads to the continued packaging of numerous hybrids into the phi29 protein shell.Nucleic acids research · 2025Article
- Revolving hexameric ATPases as asymmetric motors to translocate double-stranded DNA genome along one strand.iScience · 2023Review
- CryoEM structure and assembly mechanism of a bacterial virus genome gatekeeper.Nature communications · 2022Article
- Cryo-EM Structure of a Kinetically Trapped Dodecameric Portal Protein from the Pseudomonas-phage PaP3.Journal of molecular biology · 2022Article
- The remarkable viral portal vertex: structure and a plausible model for mechanism.Current opinion in virology · 2021Review
- Structural changes in bacteriophage T7 upon receptor-induced genome ejection.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The PLB measurement for the connector in Phi29 bacteriophage reveals the function of its channel loop.Biophysical journal · 2021Article
- A viral genome packaging motor transitions between cyclic and helical symmetry to translocate dsDNA.Science advances · 2021Article
- Cryo-EM structure in situ reveals a molecular switch that safeguards virus against genome loss.eLife · 2020Article
- Portal Protein: The Orchestrator of Capsid Assembly for the dsDNA Tailed Bacteriophages and Herpesviruses.Annual review of virology · 2019Review
- Structures of T7 bacteriophage portal and tail suggest a viral DNA retention and ejection mechanism.Nature communications · 2019Article
- Controlling the Revolving and Rotating Motion Direction of Asymmetric Hexameric Nanomotor by Arginine Finger and Channel Chirality.ACS nano · 2019Review
- May the Road Rise to Meet You: DNA Deformation May Drive DNA Translocation.Biophysical journal · 2019Article
- Nucleotide-dependent DNA gripping and an end-clamp mechanism regulate the bacteriophage T4 viral packaging motor.Nature communications · 2018Article
- Porphyrin-Assisted Docking of a Thermophage Portal Protein into Lipid Bilayers: Nanopore Engineering and Characterization.ACS nano · 2017Article
- Portal protein functions akin to a DNA-sensor that couples genome-packaging to icosahedral capsid maturation.Nature communications · 2017Article
- Three-step channel conformational changes common to DNA packaging motors of bacterial viruses T3, T4, SPP1, and Phi29.Virology · 2017Article
- Forces from the Portal Govern the Late-Stage DNA Transport in a Viral DNA Packaging Nanomotor.Biophysical journal · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Double-stranded DNA bacteriophages and their eukaryotic virus counterparts have 12-fold head-tail connector assemblages embedded at a unique capsid vertex. This vertex is the site of assembly of the DNA packaging motor, and the connector has a central channel through which viral DNA passes during genome packaging and subsequent host infection. Crystal structures of connectors from different phages reveal either disordered residues or structured loops that project into the connector channel. Given the proximity to the translocating DNA substrate, these loops have been proposed to play a role in DNA packaging. Previous models have proposed structural motions in either the packaging ATPase or the connector channel loops as the driving force that translocates the DNA into the prohead. Here, we mutate the channel loops of the Bacillus subtilis bacteriophage φ29 connector and show that these loops have no active role in translocation of DNA. Instead, they appear to have an essential function near the end of packaging, acting to retain the packaged DNA in the head in preparation for motor detachment and subsequent tail assembly and virion completion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.