ReviewMicrobial cell factories2011
Strain engineering for improved expression of recombinant proteins in bacteria.
Review in Microbial cell factories, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
80 citing papers in PubMed, 198 citations in OpenAlex.
- Nucleases and Their Inhibitors: Exploring Biological Roles, Industrial Applications, and Challenges in Heterologous Expression.Biotechnology journal · 2026Review
- An Optimized and Systematically Integrated Expression, Extraction, and Purification Strategy Enabling Efficient Production of TCR-Like Antibodies in Escherichia coli.Current microbiology · 2026Article
- Hidden Activities of Tyrosine Phenol-Lyase and Tryptophan Indole-Lyase: Recombinant PLP-Dependent C-C Lyases as New Biocatalysts for Antimicrobial Thiosulfinate Generation.Pharmaceuticals (Basel, Switzerland) · 2026Article
- A Chromosomally Integrated T7 RNA Polymerase Enables T7-Derived Expression inJournal of microbiology and biotechnology · 2025Article
- Unlocking the power of antimicrobial peptides: advances in production, optimization, and therapeutics.Frontiers in cellular and infection microbiology · 2025Review
- Rapid and Specific Detection ofACS applied bio materials · 2024Article
- Modulating bacterial function utilizing A knowledge base of transcriptional regulatory modules.Nucleic acids research · 2024Article
- Vibrational imaging of metabolites for improved microbial cell strains.Journal of biomedical optics · 2024Review
- A non-viral DNA delivery system consisting of multifunctional chimeric peptide fused with zinc-finger protein.iScience · 2024Article
- Construction of recombinant Lactococcus expressing thymosin and interferon fusion protein and its application as an immune adjuvant.Microbial cell factories · 2024Article
- Escherichia coli strains with precise domain deletions in the ribonuclease RNase E can achieve greatly enhanced levels of membrane protein production.Protein science : a publication of the Protein Society · 2024Article
- Implementation of Fluorescent-Protein-Based Quantification Analysis in L-Form Bacteria.Bioengineering (Basel, Switzerland) · 2024Article
- Heterologous expression of a deacetylase and its application in L-glufosinate preparation.Bioprocess and biosystems engineering · 2023Article
- High-throughput process development from gene cloning to protein production.Microbial cell factories · 2023Review
- Factors involved in heterologous expression of proteins in E. coli host.Archives of microbiology · 2023Review
- Steps toward a digital twin for functional food production with increased health benefits.Current research in food science · 2023Article
- Current state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems.Frontiers in pharmacology · 2023Review
- Second-GenerationACS synthetic biology · 2022Article
- Metabolic Fingerprint Analysis of CytochromeFrontiers in microbiology · 2022Article
- Comparative expression of soluble, active human kinases in specialized bacterial strains.PloS one · 2022Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein expression in Escherichia coli represents the most facile approach for the preparation of non-glycosylated proteins for analytical and preparative purposes. So far, the optimization of recombinant expression has largely remained a matter of trial and error and has relied upon varying parameters, such as expression vector, media composition, growth temperature and chaperone co-expression. Recently several new approaches for the genome-scale engineering of E. coli to enhance recombinant protein expression have been developed. These methodologies now enable the generation of optimized E. coli expression strains in a manner analogous to metabolic engineering for the synthesis of low-molecular-weight compounds. In this review, we provide an overview of strain engineering approaches useful for enhancing the expression of hard-to-produce proteins, including heterologous membrane proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.