ArticleBMC neuroscience2011
Endogenous amyloidogenesis in long-term rat hippocampal cell cultures.
Article in BMC neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Zebrafish as a Model for Multiple Sclerosis.Biomedicines · 2024Review
- Preferred Endocytosis of Amyloid Precursor Protein from Cholesterol-Enriched Lipid Raft Microdomains.Molecules (Basel, Switzerland) · 2020Article
- Inhibition of the Dead Box RNA Helicase 3 Prevents HIV-1 Tat and Cocaine-Induced Neurotoxicity by Targeting Microglia Activation.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2020Article
- [Activation of PPARγ pathway enhances cellular anti-oxidant capacity to protect long-term cultured primary rat neural cells from apoptosis].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2019Article
- Dose-dependent neurocognitive deficits following postnatal day 10 HIV-1 viral protein exposure: Relationship to hippocampal anatomy parameters.International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience · 2018Article
- rhEPO Enhances Cellular Anti-oxidant Capacity to Protect Long-Term Cultured Aging Primary Nerve Cells.Journal of molecular neuroscience : MN · 2017Article
- Article
- miR-204 downregulates EphB2 in aging mouse hippocampal neurons.Aging cell · 2016Article
- HIV-1 Tat and cocaine mediated synaptopathy in cortical and midbrain neurons is prevented by the isoflavone Equol.Frontiers in microbiology · 2015Article
- Synaptodendritic recovery following HIV Tat exposure: neurorestoration by phytoestrogens.Journal of neurochemistry · 2014Article
- Mitochondrial dysfunction and calcium deregulation by the RanBP9-cofilin pathway.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- HIV-1 Tat protein variants: critical role for the cysteine region in synaptodendritic injury.Experimental neurology · 2013Article
- Mitochondrial quality, dynamics and functional capacity in Parkinson's disease cybrid cell lines selected for Lewy body expression.Molecular neurodegeneration · 2013Article
- Towards neuronal organoids: a method for long-term culturing of high-density hippocampal neurons.PloS one · 2013Article
- D1/NMDA receptors and concurrent methamphetamine+ HIV-1 Tat neurotoxicity.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2012Article
- Loss of glutathione homeostasis associated with neuronal senescence facilitates TRPM2 channel activation in cultured hippocampal pyramidal neurons.Molecular brain · 2012Article
- Long-term culture of astrocytes attenuates the readily releasable pool of synaptic vesicles.PloS one · 2012Article
- Amyloid triggers extensive cerebral angiogenesis causing blood brain barrier permeability and hypervascularity in Alzheimer's disease.PloS one · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
backgroundLong-term primary neuronal cultures are a useful tool for the investigation of biochemical processes associated with neuronal senescence. Improvements in available technology make it possible to observe maturation of neural cells isolated from different regions of the rodent brain over a prolonged period in vitro. Existing experimental evidence suggests that cellular aging occurs in mature, long-term, primary neuronal cell cultures. However, detailed studies of neuronal development in vitro are needed to demonstrate the validity of long-term cell culture-based models for investigation of the biochemical mechanisms of in vitro neuronal development and senescence.
resultsIn the current study, neuron-enriched hippocampal cell cultures were used to analyze the differentiation and degeneration of hippocampal neurons over a two month time period. The expression of different neuronal and astroglial biomarkers was used to determine the cytochemical characteristics of hippocampal cells in long-term cultures of varying ages. It was observed that the expression of the intermediate filament nestin was absent from cultures older than 21 days in vitro (DIV), and the expression of neuronal or astrocytic markers appeared to replace nestin. Additionally, morphological evaluations of neuronal integrity and Hoescht staining were used to assess the cellular conditions in the process of hippocampal culture development and aging. It was found that there was an increase in endogenous production of Aβ(1-42) and an increase in the accumulation of Congo Red-binding amyloidal aggregates associated with the aging of neurons in primary culture. In vitro changes in the morphology of co-existing astrocytes and cell culture age-dependent degeneration of neurodendritic network resemble features of in vivo brain aging at the cellular level.
conclusionIn conclusion, this study suggests that long-term primary CNS culture is a viable model for the study of basic mechanisms and effective methods to decelerate the process of neuronal senescence.
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