ArticleBiochemistry2011
Probing oxygen activation sites in two flavoprotein oxidases using chloride as an oxygen surrogate.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 80 citations in OpenAlex.
- Ten years of integrative carbohydrate structure validation with Privateer.Glycobiology · 2026Review
- Structural and functional analysis of Bacillus sarcosine oxidase and its activity toward cyclic imino acids.FEBS open bio · 2025Article
- Understanding the riddle of amine oxidase flavoenzyme reactivity on the stereoisomers of N-methyl-dopa and N-methyl-tyrosine.Journal of molecular recognition : JMR · 2024Article
- Online carbohydrate 3D structure validation with the Privateer web app.Acta crystallographica. Section F, Structural biology communications · 2024Article
- Unveiling the kinetic versatility of aryl-alcohol oxidases with different electron acceptors.Frontiers in bioengineering and biotechnology · 2024Article
- Monitoring carbohydrate 3D structure quality with theBeilstein journal of organic chemistry · 2024Article
- Bioengineering a glucose oxidase nanosensor for near-infrared continuous glucose monitoring.Nanoscale advances · 2022Article
- Glucose Oxidase, an Enzyme "Ferrari": Its Structure, Function, Production and Properties in the Light of Various Industrial and Biotechnological Applications.Biomolecules · 2022Review
- Tuning of pThe Journal of biological chemistry · 2020Article
- Oxidative cyclization ofThe Journal of biological chemistry · 2018Article
- Enzymatic control of dioxygen binding and functionalization of the flavin cofactor.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The ins and outs of vanillyl alcohol oxidase: Identification of ligand migration paths.PLoS computational biology · 2017Article
- Shuffling Active Site Substate Populations Affects Catalytic Activity: The Case of Glucose Oxidase.ACS catalysis · 2017Article
- Comparative transcriptome analysis reveals different strategies for degradation of steam-exploded sugarcane bagasse by Aspergillus niger and Trichoderma reesei.BMC genomics · 2017Article
- X-ray structures of fructosyl peptide oxidases revealing residues responsible for gating oxygen access in the oxidative half reaction.Scientific reports · 2017Article
- Oxygen Activation at the Active Site of a Fungal Lytic Polysaccharide Monooxygenase.Angewandte Chemie (International ed. in English) · 2017Article
- Kinetics of O2 Entry and Exit in Monomeric Sarcosine Oxidase via Markovian Milestoning Molecular Dynamics.Journal of chemical theory and computation · 2016Article
- Linear and Branched PEIs (Polyethylenimines) and Their Property Space.International journal of molecular sciences · 2016Article
- Structural analysis of fungus-derived FAD glucose dehydrogenase.Scientific reports · 2015Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
A single basic residue above the si-face of the flavin ring is the site of oxygen activation in glucose oxidase (GOX) (His516) and monomeric sarcosine oxidase (MSOX) (Lys265). Crystal structures of both flavoenzymes exhibit a small pocket at the oxygen activation site that might provide a preorganized binding site for superoxide anion, an obligatory intermediate in the two-electron reduction of oxygen. Chloride binds at these polar oxygen activation sites, as judged by solution and structural studies. First, chloride forms spectrally detectable complexes with GOX and MSOX. The protonated form of His516 is required for tight binding of chloride to oxidized GOX and for rapid reaction of reduced GOX with oxygen. Formation of a binary MSOX·chloride complex requires Lys265 and is not observed with Lys265Met. Binding of chloride to MSOX does not affect the binding of a sarcosine analogue (MTA, methylthioactetate) above the re-face of the flavin ring. Definitive evidence is provided by crystal structures determined for a binary MSOX·chloride complex and a ternary MSOX·chloride·MTA complex. Chloride binds in the small pocket at a position otherwise occupied by a water molecule and forms hydrogen bonds to four ligands that are arranged in approximate tetrahedral geometry: Lys265:NZ, Arg49:NH1, and two water molecules, one of which is hydrogen bonded to FAD:N5. The results show that chloride (i) acts as an oxygen surrogate, (ii) is an effective probe of polar oxygen activation sites, and (iii) provides a valuable complementary tool to the xenon gas method that is used to map nonpolar oxygen-binding cavities.
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