ArticleJournal of neuroscience research2011
Reduced gap junctional communication among astrocytes in experimental diabetes: contributions of altered connexin protein levels and oxidative-nitrosative modifications.
Article in Journal of neuroscience research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Astrocytes in systemic regulation of blood glucose and the development of type 2 diabetes.Frontiers in cellular neuroscience · 2026Review
- NG2-Glia Cause Diabetic Blood-Brain Barrier Disruption by Secreting MMP-9.Diabetes & metabolism journal · 2026Article
- Connexin 43 is downregulated in advanced Parkinson's disease in multiple brain regions which correlates with symptoms.Scientific reports · 2025Article
- Long-Term Exposure of Cultured Astrocytes to High Glucose Impact on Their LPS-Induced Activation.International journal of molecular sciences · 2024Article
- Effects of Diabetes Mellitus-Related Dysglycemia on the Functions of Blood-Brain Barrier and the Risk of Dementia.International journal of molecular sciences · 2023Review
- PHPB Attenuated Cognitive Impairment in Type 2 Diabetic KK-Ay Mice by Modulating SIRT1/Insulin Signaling Pathway and Inhibiting Generation of AGEs.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Connexins and Glucose Metabolism in Cancer.International journal of molecular sciences · 2022Review
- Astrocytic responses to high glucose impair barrier formation in cerebral microvessel endothelial cells.American journal of physiology. Regulatory, integrative and comparative physiology · 2022Article
- Review
- Fraxin Promotes the Activation of Nrf2/ARE Pathway via Increasing the Expression of Connexin43 to Ameliorate Diabetic Renal Fibrosis.Frontiers in pharmacology · 2022Article
- Ultrastructure of gap junction and Cx43 expression in gastric cancer tissues of the patients.Archives of medical science : AMS · 2020Article
- Connexin channel and its role in diabetic retinopathy.Progress in retinal and eye research · 2017Review
- Methylglyoxal Induces Changes in the Glyoxalase System and Impairs Glutamate Uptake Activity in Primary Astrocytes.Oxidative medicine and cellular longevity · 2017Article
- Regulation of gap junction channels and hemichannels by phosphorylation and redox changes: a revision.BMC cell biology · 2016Review
- Glucose Tightly Controls Morphological and Functional Properties of Astrocytes.Frontiers in aging neuroscience · 2016Article
- Glutamate mediated astrocytic filtering of neuronal activity.PLoS computational biology · 2014Article
- Diabetes Mellitus and Blood-Brain Barrier Dysfunction: An Overview.Journal of pharmacovigilance · 2014Article
- Hypothalamic astroglial connexins are required for brain glucose sensing-induced insulin secretion.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2014Article
- Astrocytic energetics during excitatory neurotransmission: What are contributions of glutamate oxidation and glycolysis?Neurochemistry international · 2013Review
- The unfolded protein response to endoplasmic reticulum stress in cultured astrocytes and rat brain during experimental diabetes.Neurochemistry international · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Experimental diabetes increases production of reactive oxygen-nitrogen species and inhibits astrocytic gap junctional communication in tissue culture and brain slices from streptozotocin (STZ)-diabetic rats by unidentified mechanisms. Relative connexin (Cx) protein levels were assessed by Western blotting using extracts from cultured astrocytes grown in high (25 mmol/liter) or low (5.5 mmol/liter) glucose for 2-3 weeks and STZ-diabetic rat brain. Chemiluminescent signals for diabetic samples were normalized to those of controls on the same blot and same protein load. Growth in high glucose did not alter relative Cx26 level, whereas Cx30 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were reduced by ∼30%, and Cx43 increased ∼1.9-fold. In the inferior colliculus of STZ-diabetic rats, Cx30 and Cx43 levels in three of four rats were half those of controls, whereas GAPDH and actin were unaffected. Diabetes did not affect levels of Cx30, Cx43, or GAPDH in cerebral cortex, but actin level rose 24%. Cx43 was predominantly phosphorylated in control and diabetic samples, so the reduced dye transfer is not due to overall dephosphorylation of Cx43. Astrocytic growth in high glucose reduced the dye-labeled area by 75%, but 10 min of treatment with dithiothreitol restored normal dye transfer. In contrast, nitric oxide donors inhibited dye transfer among astrocytes grown in low glucose by 50-65% within 1 hr. Thus, modifications arising from oxidative-nitrosative stress, not altered connexin levels, may underlie the reduced dye transfer among severely hyperglycemic cultured astrocytes, whereas both oxidative-nitrosative stress and regionally selective down-regulation of connexin protein content may affect gap junctional communication in the brains of STZ-diabetic rats.
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