ArticleInternational orthopaedics2012
Osteogenic differentiation is synergistically influenced by osteoinductive treatment and direct cell-cell contact between murine osteoblasts and mesenchymal stem cells.
Article in International orthopaedics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Induction of Mesenchymal Stem Cell and Preosteoblast Differentiation: A Comparative Study Between Conditioned Medium and In Vitro Coculture.International journal of dentistry · 2026Article
- A Scaffold-Based 3D Culture Model Including Selected Osteoprogenitors for Bone Regeneration With Controlled Morphology.Stem cells international · 2026Article
- Histological evaluation of effects of krill oil and eucalyptus oil on bone healing in rats.Journal of Taibah University Medical Sciences · 2025Article
- Revolutionizing bone regeneration and wound healing: Mechanical stromal vascular fraction and hyaluronic acid in a mouse calvarial defect model.Frontiers in cell and developmental biology · 2025Article
- Extracellular derivatives for bone metabolism.Journal of advanced research · 2024Review
- Effects of Different No-Ozone Cold Plasma Treatment Methods on Mouse Osteoblast Proliferation and Differentiation.Medicina (Kaunas, Lithuania) · 2024Article
- Exosome mediated biological functions within skeletal microenvironment.Frontiers in bioengineering and biotechnology · 2022Review
- Growth characteristics of human bone marrow mesenchymal stromal cells at cultivation on synthetic polyelectrolyte nanofilmsHeliyon · 2021Article
- Role of microRNA-335 carried by bone marrow mesenchymal stem cells-derived extracellular vesicles in bone fracture recovery.Cell death & disease · 2021Article
- Nascent osteoblast matrix inhibits osteogenesis of human mesenchymal stem cells in vitro.Stem cell research & therapy · 2015Article
- The role of growth factors in stem cell-directed chondrogenesis: a real hope for damaged cartilage regeneration.International orthopaedics · 2015Review
- Evaluation of intervertebral disc regeneration with implantation of bone marrow mesenchymal stem cells (BMSCs) using quantitative T2 mapping: a study in rabbits.International orthopaedics · 2015Article
- Efficient derivation of osteoprogenitor cells from induced pluripotent stem cells for bone regeneration.International orthopaedics · 2014Article
- Bone marrow derived stem cells in joint and bone diseases: a concise review.International orthopaedics · 2014Review
- Role of mesenchymal stem cells in bone regeneration and fracture repair: a review.International orthopaedics · 2013Review
- Comparative characteristics of porous bioceramics for an osteogenic response in vitro and in vivo.PloS one · 2013Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe present study was designed to address whether osteoblasts play a synergistic role in promoting mesenchymal stem cell (MSC) osteogenesis in a direct cell-cell contact co-culture model.
methodsMurine C3H10T1/2 and MC3T3-E1 cell lines were mixed and plated onto 12-well culture plates and co-cultured at various ratios of initial cell densities. To compare the possible improvement on osteogenic differentiation, co-culture cells were served with or without osteogenic supplements in culture medium.
resultsWeak osteogenesis was induced in MSCs co-cultured in an untreated medium with different ratios of osteoblasts. An osteoblast-dependent increase in osteogenic gene expression of Runx2, type I collagen, and osteocalcin was observed over time. Moreover, both alkaline phosphatase (ALP) activity and calcium deposition were distinctly enhanced at levels that were proportional to the quantity of osteoblasts in the culture. The increases in mRNA expression and ALP activity were greater in co-cultures treated with osteogenic supplements than in untreated cultures. However, the production of ALP activity followed by a distinct matrix mineralization was lower in osteogenic-treated cultures containing greater numbers of osteoblasts. This suggests that a higher density of osteoblasts may lead to weak osteogenesis of MSCs by direct cell-cell contact co-culture in an untreated environment. Furthermore, additional osteogenic supplements may act synergistically with osteoblasts to accelerate matrix mineralization by reducing the process of osteogenic differentiation in osteogenic treated co-cultures.
conclusionsThe present work may improve the understanding of MSC osteogenesis and may provide benefits for regenerative medicine.
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