ArticleParasitology international2011
RNA interference targeting cathepsin B of the carcinogenic liver fluke, Opisthorchis viverrini.
Article in Parasitology international, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Fluke-borne viruses are a risk factor for diseases caused by carcinogenic trematodes.bioRxiv : the preprint server for biology · 2025Article
- Transgenesis in parasitic helminths: a brief history and prospects for the future.Parasites & vectors · 2022Review
- RNA interference dynamics in juvenile Fasciola hepatica are altered during in vitro growth and development.International journal for parasitology. Drugs and drug resistance · 2020Article
- Opisthorchis viverrini Proteome and Host-Parasite Interactions.Advances in parasitology · 2018Review
- RNA interference in Fasciola gigantica: Establishing and optimization of experimental RNAi in the newly excysted juveniles of the fluke.PLoS neglected tropical diseases · 2017Article
- Conservation and diversification of small RNA pathways within flatworms.BMC evolutionary biology · 2017Article
- Liver fluke infection and cholangiocarcinoma: a review.Parasitology research · 2017Review
- Stimulating Neoblast-Like Cell Proliferation in Juvenile Fasciola hepatica Supports Growth and Progression towards the Adult Phenotype In Vitro.PLoS neglected tropical diseases · 2016Article
- Identification and characterization of Clonorchis sinensis cathepsin B proteases in the pathogenesis of clonorchiasis.Parasites & vectors · 2015Article
- Carcinogenic Parasite Secretes Growth Factor That Accelerates Wound Healing and Potentially Promotes Neoplasia.PLoS pathogens · 2015Article
- Suppression of Ov-grn-1 encoding granulin of Opisthorchis viverrini inhibits proliferation of biliary epithelial cells.Experimental parasitology · 2015Article
- Transient transgenesis of the tapeworm Taenia crassiceps.SpringerPlus · 2015Article
- Cathepsin B Cysteine Proteinase is Essential for the Development and Pathogenesis of the Plant Parasitic Nematode Radopholus similis.International journal of biological sciences · 2015Article
- RNAi dynamics in Juvenile Fasciola spp. Liver flukes reveals the persistence of gene silencing in vitro.PLoS neglected tropical diseases · 2014Article
- Characterization of the secreted cathepsin B cysteine proteases family of the carcinogenic liver fluke Clonorchis sinensis.Parasitology research · 2014Article
- The Opisthorchis viverrini genome provides insights into life in the bile duct.Nature communications · 2014Article
- RNAi-mediated silencing of enolase confirms its biological importance in Clonorchis sinensis.Parasitology research · 2014Article
- The tumorigenic liver fluke Opisthorchis viverrini--multiple pathways to cancer.Trends in parasitology · 2012Review
- Molecular characterization of a tetraspanin from the human liver fluke, Opisthorchis viverrini.PLoS neglected tropical diseases · 2012Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
Abstract
Functional genomics have not been reported for Opisthorchis viverrini or the related fish-borne fluke, Clonorchis sinensis. Here we describe the introduction by square wave electroporation of Cy3-labeled small RNA into adult O. viverrini worms. Adult flukes were subjected to square wave electroporation employing a single pulse for 20 ms of 125V in the presence of 50 μg/ml of Cy3-siRNA. The parasites tolerated this manipulation and, at 24 and 48 h after electroporation, fluorescence from the Cy3-siRNA was evident throughout the parenchyma of the worms, with strong fluorescence evident in the guts and reproductive organs of the adult worms. Second, other worms were treated using the same electroporation settings with double stranded RNA targeting an endogenous papain-like cysteine protease, cathepsin B. This manipulation resulted in a significant reduction in specific mRNA levels encoding cathepsin B, and a significant reduction in cathepsin B activity against the diagnostic peptide, Z-Arg-Arg-AMC. This appears to be the first report of introduction of reporter genes into O. viverrini and the first report of experimental RNA interference (RNAi) in this fluke. The findings indicated the presence of an intact RNAi pathway in these parasites which, in turn, provides an opportunity to probe gene functions in this neglected tropical disease pathogen.
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Registered trials
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