ArticleJournal of immunological methods2011
Antigen profiles for the quantitative assessment of eosinophils in mouse tissues by flow cytometry.
Article in Journal of immunological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 46 citations in OpenAlex.
- Spleen and peripheral blood immunopathology in an outbred model of adult-stage murine schistosomiasis.Frontiers in immunology · 2025Article
- CRL4B complex-mediated H2AK119 monoubiquitination restrains Th1 and Th2 cell differentiation.Cell death and differentiation · 2023Article
- Influenza A virus directly modulates mouse eosinophil responses.Journal of leukocyte biology · 2020Article
- Frontline Science: Cytokine-mediated developmental phenotype of mouse eosinophils: IL-5-associated expression of the Ly6G/Gr1 surface Ag.Journal of leukocyte biology · 2020Article
- Mimicking Antigen-Driven Asthma in Rodent Models-How Close Can We Get?Frontiers in immunology · 2020Review
- Microbial Regulation of Enteric Eosinophils and Its Impact on Tissue Remodeling and Th2 Immunity.Frontiers in immunology · 2020Article
- Opposing roles of eosinophils in cancer.Cancer immunology, immunotherapy : CII · 2019Review
- Impact of eosinophil-peroxidase (EPX) deficiency on eosinophil structure and function in mouse airways.Journal of leukocyte biology · 2019Article
- Cysteinyl leukotriene receptor 1 expression identifies a subset of neutrophils during the antiviral response that contributes to postviral atopic airway disease.The Journal of allergy and clinical immunology · 2018Article
- FACS isolation of live mouse eosinophils at high purity via a protocol that does not target Siglec F.Journal of immunological methods · 2018Article
- Novel adherent CD11bOncotarget · 2018Article
- Eosinophil persistence in vivo and sustained viability ex vivo in response to respiratory challenge with fungal allergens.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2018Article
- SiglecF+Gr1hi eosinophils are a distinct subpopulation within the lungs of allergen-challenged mice.Journal of leukocyte biology · 2017Article
- IL-33 Precedes IL-5 in Regulating Eosinophil Commitment and Is Required for Eosinophil Homeostasis.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Lung-resident eosinophils represent a distinct regulatory eosinophil subset.The Journal of clinical investigation · 2016Article
- Signaling via pattern recognition receptors NOD2 and TLR2 contributes to immunomodulatory control of lethal pneumovirus infection.Antiviral research · 2016Article
- Immortalized MH-S cells lack defining features of primary alveolar macrophages and do not support mouse pneumovirus replication.Immunology letters · 2016Article
- Transcription Factor Repertoire of Homeostatic Eosinophilopoiesis.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Granulocyte Macrophage Colony-Stimulating Factor-Activated Eosinophils Promote Interleukin-23 Driven Chronic Colitis.Immunity · 2015Article
- Macrophages Subvert Adaptive Immunity to Urinary Tract Infection.PLoS pathogens · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Much of our current understanding of eosinophil-associated pathologies has developed from the use of mouse models. While mouse eosinophils can be readily detected by flow cytometric methods, most studies do not document the efficiency of this process compared to direct counting of stained cells. Our intent was to address this knowledge gap by identifying one or more eosinophil-specific antigen profiles that yielded flow cytometric data that was statistically consistent with direct counts. We found that anti-CD193 (CCR3) and anti-CD125 (IL-5Rα) antibodies were effective at detecting eosinophils in bone marrow of interleukin-5 transgenic mice, but these antibodies under-reported the percent positive cells. In contrast, anti-Siglec F alone or in combination with anti-CD45 can be used for the quantitative detection of eosinophils in mouse bone marrow and spleen. The antigen profile CD45(+)SiglecF(+)CD11c(-) was effective at detecting eosinophils in the lung as well as bone marrow and spleen, and the results obtained correlated with direct morphometric counts under all conditions evaluated (r(2)=0.98-0.99). To the best of our knowledge, this is the first systematic analysis presenting definitive correlations between percent eosinophils detected by cell surface markers and direct counting of stained cells in multiple tissues and at varying degrees of eosinophilia.
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