ArticleMolecular microbiology2011
Mutations in the nucleotide binding pocket of MreB can alter cell curvature and polar morphology in Caulobacter.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 68 citations in OpenAlex.
- RodZ acts through MreBCD to activate the elongasome inJournal of bacteriology · 2026Article
- Alanine-scanning mutagenesis library of MreB reveals distinct roles for regulating cell shape and viability.PLoS genetics · 2026Article
- Mechanics ofMolecular biology of the cell · 2025Article
- Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus.Nature communications · 2024Article
- Enzyme 1 of the phosphoenolpyruvate:sugar phosphotransferase system is involved in resistance to MreB disruption in wild-type and ∆envC cells.Molecular microbiology · 2022Article
- The material properties of a bacterial-derived biomolecular condensate tune biological function in natural and synthetic systems.Nature communications · 2022Article
- Filament organization of the bacterial actin MreB is dependent on the nucleotide state.The Journal of cell biology · 2022Article
- Elongation at Midcell in Preparation of Cell Division Requires FtsZ, but Not MreB nor PBP2 inFrontiers in microbiology · 2021Article
- AimB Is a Small Protein Regulator of Cell Size and MreB Assembly.Biophysical journal · 2020Article
- Chiral twisting in a bacterial cytoskeletal polymer affects filament size and orientation.Nature communications · 2020Article
- Conservation of conformational dynamics across prokaryotic actins.PLoS computational biology · 2019Article
- Genomic correlates of extraintestinal infection are linked with changes in cell morphology in Campylobacter jejuni.Microbial genomics · 2019Article
- Cytological analysis and structural quantification of FtsZ1-2 and FtsZ2-1 network characteristics in Physcomitrella patens.Scientific reports · 2018Article
- RodZ modulates geometric localization of the bacterial actin MreB to regulate cell shape.Nature communications · 2018Article
- Is Longitudinal Division in Rod-Shaped Bacteria a Matter of Swapping Axis?Frontiers in microbiology · 2018Review
- Sizing up the bacterial cell cycle.Nature reviews. Microbiology · 2017Review
- Shapeshifting to Survive: Shape Determination and Regulation in Caulobacter crescentus.Trends in microbiology · 2017Review
- Rapid, precise quantification of bacterial cellular dimensions across a genomic-scale knockout library.BMC biology · 2017Article
- MreB Orientation Correlates with Cell Diameter in Escherichia coli.Biophysical journal · 2016Article
- YodL and YisK Possess Shape-Modifying Activities That Are Suppressed by Mutations in Bacillus subtilis mreB and mbl.Journal of bacteriology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The maintenance of cell shape in Caulobacter crescentus requires the essential gene mreB, which encodes a member of the actin superfamily and the target of the antibiotic, A22. We isolated 35 unique A22-resistant Caulobacter strains with single amino acid substitutions near the nucleotide binding site of MreB. Mutations that alter cell curvature and mislocalize the intermediate filament crescentin cluster on the back surface of MreB's structure. Another subset have variable cell widths, with wide cell bodies and actively growing thin extensions of the cell poles that concentrate fluorescent MreB. We found that the extent to which MreB localization is perturbed is linearly correlated with the development of pointed cell poles and variable cell widths. Further, we find that a mutation to glycine of two conserved aspartic acid residues that are important for nucleotide hydrolysis in other members of the actin superfamily abolishes robust midcell recruitment of MreB but supports a normal rate of growth. These mutant strains provide novel insight into how MreB's protein structure, subcellular localization, and activity contribute to its function in bacterial cell shape.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.