ArticleMolecular microbiology2011
Genetic analysis of selenocysteine biosynthesis in the archaeon Methanococcus maripaludis.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Massive Cultivation ofACS omega · 2026Article
- Model Organisms To Study Methanogenesis, a Uniquely Archaeal Metabolism.Journal of bacteriology · 2023Review
- Methanogens: biochemical background and biotechnological applications.AMB Express · 2018Review
- Structural basis for tRNA-dependent cysteine biosynthesis.Nature communications · 2017Article
- Genetic code flexibility in microorganisms: novel mechanisms and impact on physiology.Nature reviews. Microbiology · 2015Review
- Comparative genomics reveals new candidate genes involved in selenium metabolism in prokaryotes.Genome biology and evolution · 2015Article
- Biochemical functional predictions for protein structures of unknown or uncertain function.Computational and structural biotechnology journal · 2015Review
- Carbon source-dependent expansion of the genetic code in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Selenomodification of tRNA in archaea requires a bipartite rhodanese enzyme.FEBS letters · 2012Article
- Overview of the genetic tools in the Archaea.Frontiers in microbiology · 2012Article
- Elemental economy: microbial strategies for optimizing growth in the face of nutrient limitation.Advances in microbial physiology · 2012Review
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5 authors at 1 institution in 1 country.
Funding
Abstract
In Archaea selenocysteine (Sec) is synthesized in three steps. First seryl-tRNA synthetase acylates tRNA(Sec) with serine to generate Ser-tRNA(Sec). Then phosphoseryl-tRNA(Sec) kinase (PSTK) forms Sep-tRNA(Sec) , which is converted to Sec-tRNA(Sec) by Sep-tRNA:Sec-tRNA synthase (SepSecS) in the presence of selenophosphate produced by selenophosphate synthetase (SelD). A complete in vivo analysis of the archaeal Sec biosynthesis pathway is still unavailable, and the existence of a redundant pathway or of a rescue mechanism based on the conversion of Sep-tRNA(Sec) to Cys-tRNA(Sec) during selenium starvation, cannot be excluded. Here we present a mutational analysis of Sec biosynthesis in Methanococcus maripaludis strain Mm900. Sec formation is abolished upon individually deleting the genes encoding SelD, PSTK or SepSecS; the resulting mutant strains could no longer grow on formate while growth with H(2) + CO(2) remained unaffected. However, deletion of the PSTK and SepSecS genes was not possible unless the selenium-free [NiFe]-hydrogenases Frc and Vhc were expressed. This required the prior deletion of either the gene encoding SelD or that of HrsM, a LysR-type regulator suppressing transcription of the frc and vhc operons in the presence of selenium. These results show that M. maripaludis Mm900 is facultatively selenium-dependent with a single pathway of Sec-tRNA(Sec) formation.
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