ArticlePlant physiology2011
A versatile set of ligation-independent cloning vectors for functional studies in plants.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 151 citations in OpenAlex.
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- Random epigenetic inactivation of the X-chromosomal HaMSter gene causes sex ratio distortion in persimmon.Nature plants · 2024Article
- H3K9 methylation regulates heterochromatin silencing through incoherent feedforward loops.Science advances · 2024Article
- CDC48A, an interactor of WOX2, is required for embryonic patterning in Arabidopsis thaliana.Plant cell reports · 2024Article
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- Transcription-coupled and epigenome-encoded mechanisms direct H3K4 methylation.Nature communications · 2022Article
- Transcriptome Analysis of Fusarium-Tomato Interaction Based on an Updated Genome Annotation ofJournal of fungi (Basel, Switzerland) · 2022Article
- All-in-one: a robust fluorescent fusion protein vector toolbox for protein localization and BiFC analyses in plants.Plant biotechnology journal · 2022Article
- Genome-wide cis-decoding for expression design in tomato using cistrome data and explainable deep learning.The Plant cell · 2022Article
- Reinvention of hermaphroditism via activation of a RADIALIS-like gene in hexaploid persimmon.Nature plants · 2022Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With plant molecular biology entering the omics era, there is a need for simple cloning strategies that allow high throughput to systematically study the expression and function of large numbers of genes. Such strategies would facilitate the analysis of gene (sub)families and/or sets of coexpressed genes identified by transcriptomics. Here, we provide a set of 34 ligation-independent cloning (LIC) binary vectors for expression analysis, protein localization studies, and misexpression that will be made freely available. This set of plant LIC vectors offers a fast alternative to standard cloning strategies involving ligase or recombination enzyme technology. We demonstrate the use of this strategy and our new vectors by analyzing the expression domains of genes belonging to two subclades of the basic helix-loop-helix transcription factor family. We show that neither the closest homologs of TARGET OF MONOPTEROS7 (TMO7/ATBS1) nor the members of the ATBS1 INTERACTING FACTOR subclade of putative TMO7 interactors are expressed in the embryo and that there is very limited coexpression in the primary root meristem. This suggests that these basic helix-loop-helix transcription factors are most likely not involved in TMO7-dependent root meristem initiation.
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