ArticleJournal of clinical microbiology2011
Development of a new chlamydiales-specific real-time PCR and its application to respiratory clinical samples.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Article
- Article
- Recruitment of the cellular lipid transport protein CERT to C. psittaci inclusions regulates the timing of bacterial egress.Scientific reports · 2025Article
- Molecular detection of Chlamydiales organisms in ruminant abortions in Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- The Occurrence ofPathogens (Basel, Switzerland) · 2024Article
- Article
- Ticks and Chlamydia-Related Bacteria in Swiss Zoological Gardens Compared to in Contiguous and Distant Control Areas.Microorganisms · 2023Article
- Diversity of Chlamydiales detected in pet birds privately kept in individual homes in Japan.The Journal of veterinary medical science · 2023Article
- Pathogens associated with hospitalization due to acute lower respiratory tract infections in children in rural Ghana: a case-control study.Scientific reports · 2023Article
- Optimization and Application of Real-Time qPCR Assays in Detection and Identification of Chlamydiales in Products of Domestic Ruminant Abortion.Pathogens (Basel, Switzerland) · 2023Article
- Are ELISA and PCR Discrepancies in the Identification ofMicroorganisms · 2023Article
- A Case Study of ZoonoticOpen forum infectious diseases · 2022Article
- Chlamydial species among wild birds and livestock in the foothills of Mt. Afadjato, Ghana.The Journal of veterinary medical science · 2022Article
- Evaluation of a Multiplex Real-Time PCR Assay for DetectingDiagnostics (Basel, Switzerland) · 2022Article
- Reliable and Sensitive Nested PCR for the Detection ofMicroorganisms · 2021Article
- Bovine Abortions Revisited-Enhancing Abortion Diagnostics by 16S rDNA Amplicon Sequencing and FluorescenceFrontiers in veterinary science · 2021Article
- Nested Species Distribution Models ofApplied and environmental microbiology · 2020Article
- Molecular causes of an evolutionary shift along the parasitism-mutualism continuum in a bacterial symbiont.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Prevalence and phylogeny of Chlamydiae and hemotropic mycoplasma species in captive and free-living bats.BMC microbiology · 2020Article
- Isolation of Tetracycline-ResistantAntibiotics (Basel, Switzerland) · 2020Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Originally composed of the single family Chlamydiaceae, the Chlamydiales order has extended considerably over the last several decades. Chlamydia-related bacteria were added and classified into six different families and family-level lineages: the Criblamydiaceae, Parachlamydiaceae, Piscichlamydiaceae, Rhabdochlamydiaceae, Simkaniaceae, and Waddliaceae. While several members of the Chlamydiaceae family are known pathogens, recent studies showed diverse associations of Chlamydia-related bacteria with human and animal infections. Some of these latter bacteria might be of medical importance since, given their ability to replicate in free-living amoebae, they may also replicate efficiently in other phagocytic cells, including cells of the innate immune system. Thus, a new Chlamydiales-specific real-time PCR targeting the conserved 16S rRNA gene was developed. This new molecular tool can detect at least five DNA copies and show very high specificity without cross-amplification from other bacterial clade DNA. The new PCR was validated with 128 clinical samples positive or negative for Chlamydia trachomatis or C. pneumoniae. Of 65 positive samples, 61 (93.8%) were found to be positive with the new PCR. The four discordant samples, retested with the original test, were determined to be negative or below detection limits. Then, the new PCR was applied to 422 nasopharyngeal swabs taken from children with or without pneumonia; a total of 48 (11.4%) samples were determined to be positive, and 45 of these were successfully sequenced. The majority of the sequences corresponded to Chlamydia-related bacteria and especially to members of the Parachlamydiaceae family.
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