ArticleThe Journal of biological chemistry2011
Caffeine promotes ultraviolet B-induced apoptosis in human keratinocytes without complete DNA repair.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 2 syntheses or guidelines pooled it, 66 citations in OpenAlex.
- Coffee, tea and caffeine intake and the risk of non-melanoma skin cancer: a review of the literature and meta-analysis.European journal of nutrition · 2017Pooled it
- Relationship between bladder cancer and total fluid intake: a meta-analysis of epidemiological evidence.World journal of surgical oncology · 2014Pooled it
- Trial
- Coffee consumption, cancer, and healthy aging: epidemiological evidence and underlying mechanisms.GeroScience · 2025Review
- Caffeine Protects Keratinocytes fromInternational journal of molecular sciences · 2024Article
- Effects of dose-dependent chronic caffeine consumption in a rat burn wound model: Histopathological and immunohistochemical evaluation.Iranian journal of basic medical sciences · 2024Article
- Photoprotective Effects ofInternational journal of molecular sciences · 2023Article
- Activation of the JNKs/ATM-p53 axis is indispensable for the cytoprotection of dermal fibroblasts exposed to UVB radiation.Cell death & disease · 2022Article
- Astaxanthin Protects Ultraviolet B-Induced Oxidative Stress and Apoptosis in Human Keratinocytes via Intrinsic Apoptotic Pathway.Annals of dermatology · 2022Article
- Hyperoside and Quercitrin inAntioxidants (Basel, Switzerland) · 2022Article
- Plant Secondary Metabolites against Skin Photodamage: Mexican Plants, a Potential Source of UV-Radiation Protectant Molecules.Plants (Basel, Switzerland) · 2022Review
- Pleiotropic Effects of Caffeine Leading to Chromosome Instability and Cytotoxicity in Eukaryotic Microorganisms.Journal of microbiology and biotechnology · 2021Review
- A Decade of Research on Coffee as an Anticarcinogenic Beverage.Oxidative medicine and cellular longevity · 2021Review
- Drug-Target Interaction Prediction Based on Adversarial Bayesian Personalized Ranking.BioMed research international · 2021Article
- Predicting keratinocyte carcinoma in patients with actinic keratosis: development and internal validation of a multivariable risk-prediction model.The British journal of dermatology · 2020Article
- Coffee, tea, caffeine, and risk of nonmelanoma skin cancer in a Chinese population: The Singapore Chinese Health Study.Journal of the American Academy of Dermatology · 2019Article
- Article
- Theophylline exhibits anti-cancer activityOncotarget · 2017Article
- A genome-wide analysis of gene-caffeine consumption interaction on basal cell carcinoma.Carcinogenesis · 2016Article
- In Vitro Chemopreventive Properties of Green Tea, Rooibos and Honeybush Extracts in Skin Cells.Molecules (Basel, Switzerland) · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In response to ultraviolet B damage, keratinocytes undergo apoptosis to eliminate damaged cells, thereby preventing tumorigenic transformation. Caffeine, the most widely consumed psychoactive substance, produces complex pharmacological actions; it has been shown to be chemopreventive in non-melamona skin cancer in mice through increasing apoptosis. Here we have investigated the molecular and cellular mechanisms in the pro-apoptotic effect of caffeine on UVB-irradiated human HaCaT keratinocytes. Pretreatment with caffeine increased UVB-induced apoptosis in HaCaT cells. Caffeine blocked UVB-induced Chk1 phosphorylation. In addition, similar to the effect of the PI3K inhibitor LY294002, caffeine also inhibited phosphorylation of AKT and up-regulation of COX-2, two critical oncogenic pathways in skin tumorigenesis. However, phosphorylation of EGFR or ERK was unaffected. Inhibiting ATR pathways by siRNA targeting ATR had little effect on UVB-induced apoptosis or AKT activation, indicating that the inhibitory effect of caffeine on apoptosis and the AKT pathway does not require the ATR pathway. Inhibiting AKT by caffeine blocked UVB-induced COX-2 up-regulation. Expression of constitutively active AKT that was not inhibited by caffeine was found to protect cells from caffeine-promoted apoptosis post-UVB irradiation, indicating that AKT is an essential inhibitory target for caffeine to promote apoptosis. Caffeine specifically sensitized cells with unrepaired DNA damage to UVB-induced apoptosis. These findings indicate that in HaCaT keratinocytes, inhibiting the AKT/COX-2 pathways through an ATR-independent pathway is a critical molecular mechanism by which caffeine promotes UVB-induced apoptosis of unrepaired keratinocytes for elimination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.