ArticleJournal of industrial microbiology & biotechnology2011
Cell physiology rather than enzyme kinetics can determine the efficiency of cytochrome P450-catalyzed C-H-oxyfunctionalization.
Article in Journal of industrial microbiology & biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Efficient Synthesis of (Frontiers in bioengineering and biotechnology · 2022Article
- Investigation of monoterpenoid resistance mechanisms in Pseudomonas putida and their consequences for biotransformations.Applied microbiology and biotechnology · 2020Article
- Maximizing Biocatalytic Cyclohexane Hydroxylation by Modulating Cytochrome P450 Monooxygenase Expression inFrontiers in bioengineering and biotechnology · 2020Article
- Pseudomonas putida KT2440 is HV1 certified, not GRAS.Microbial biotechnology · 2019Article
- Construction and Characterization of a New Recombinant Vector to Remove Sulfate Repression ofFrontiers in microbiology · 2018Article
- Biocatalytic activity of Monascus mycelia depending on physiology and high sensitivity to product concentration.AMB Express · 2017Article
- Efficient hydroxylation of 1,8-cineole with monoterpenoid-resistant recombinant Pseudomonas putida GS1.World journal of microbiology & biotechnology · 2016Article
- Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation.Microbial cell factories · 2015Article
- Synthesis of ω-hydroxy dodecanoic acid based on an engineered CYP153A fusion construct.Microbial biotechnology · 2013Article
- Proline availability regulates proline-4-hydroxylase synthesis and substrate uptake in proline-hydroxylating recombinant Escherichia coli.Applied and environmental microbiology · 2013Article
- The influence of microbial physiology on biocatalyst activity and efficiency in the terminal hydroxylation of n-octane using Escherichia coli expressing the alkane hydroxylase, CYP153A6.Microbial cell factories · 2013Article
- Outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in Escherichia coli.Applied and environmental microbiology · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell physiology is a critical factor determining the efficiency of reactions performed by microbial biocatalysts. In order to develop an efficient biotransformation procedure for the hydroxylation of (S)-limonene to (S)-perillyl alcohol by recombinant Pseudomonas putida cells harboring the cytochrome P450 monooxygenase CYP153A6, physiological parameters were optimized. The previously reported synthesis of (S)-perillyl alcohol by P. putida GPo12 was based on complex and sensitive octane feeding strategies (van Beilen et al. in Appl Environ Microbiol 71:1737-1744, 2005), indicating the pivotal role of cell physiology. In contrast to previous findings, the screening of different carbon sources showed that glycerol and citrate are suitable alternatives to octane allowing high specific limonene hydroxylation activities. The use of P. putida KT2440 as an alternative host strain and citrate as the carbon source improved practical handling and allowed a 7.5-fold increase of the specific activity (to 22.6 U g (CDW) (-1) ). In two-liquid-phase biotransformations, 4.3 g of (S)-perillyl alcohol L (tot) (-1) were produced in 24 h, representing a sixfold improvement in productivity compared to previously reported results. It is concluded that, for selective cytochrome P450-based hydrocarbon oxyfunctionalizations by means of living microbial cells, the relationship between cell physiology and the target biotransformation is crucial, and that understanding the relationship should guide biocatalyst and bioprocess design.
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