ArticlePloS one2011
Aggregatibacter actinomycetemcomitans Omp29 is associated with bacterial entry to gingival epithelial cells by F-actin rearrangement.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
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- Illuminating the oral microbiome: cellular microbiology.FEMS microbiology reviews · 2023Article
- Current concepts in the pathogenesis of periodontitis: from symbiosis to dysbiosis.Journal of oral microbiology · 2023Review
- Lactobacilli Attenuate the Effect ofFrontiers in microbiology · 2022Article
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- Nanoscale observation of PM2.5 incorporated into mammalian cells using scanning electron-assisted dielectric microscope.Scientific reports · 2021Article
- Distinct Signaling Pathways Between Human Macrophages and Primary Gingival Epithelial Cells byPathogens (Basel, Switzerland) · 2020Article
- Tools ofJournal of clinical medicine · 2019Review
- Role of OmpA1 and OmpA2 inJournal of oral microbiology · 2019Article
- Type I collagen deposition via osteoinduction ameliorates YAP/TAZ activity in 3D floating culture clumps of mesenchymal stem cell/extracellular matrix complexes.Stem cell research & therapy · 2018Article
- Modulation of microenvironment for controlling the fate of periodontal ligament cells: the role of Rho/ROCK signaling and cytoskeletal dynamics.Journal of cell communication and signaling · 2018Review
- Peptidoglycan-associated lipoprotein ofJournal of oral microbiology · 2018Article
- Xenotransplantation of interferon-gamma-pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration.Stem cell research & therapy · 2017Article
- TRAP-positive osteoclast precursors mediate ROS/NO-dependent bactericidal activity via TLR4.Free radical biology & medicine · 2016Article
- Transcriptional regulation of the Aggregatibacter actinomycetemcomitans ygiW-qseBC operon by QseB and integration host factor proteins.Microbiology (Reading, England) · 2014Article
- Differential transcription of virulence genes in Aggregatibacter actinomycetemcomitans serotypes.Journal of oral microbiology · 2013Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
The onset and progressive pathogenesis of periodontal disease is thought to be initiated by the entry of Aggregatibacter actinomycetemcomitans (Aa) into periodontal tissue, especially gingival epithelium. Nonetheless, the mechanism underlying such bacterial entry remains to be clarified. Therefore, this study aimed to investigate the possible role of Aa outer membrane protein 29 kD (Omp29), a homologue of E. coli OmpA, in promoting bacterial entry into gingival epithelial cells. To accomplish this, Omp29 expression vector was incorporated in an OmpA-deficient mutant of E. coli. Omp29(+)/OmpA(-) E. coli demonstrated 22-fold higher entry into human gingival epithelial line cells (OBA9) than Omp29(-)/OmpA(-) E. coli. While the entry of Aa and Omp29(+)/OmpA(-) E. coli into OBA9 cells were inhibited by anti-Omp29 antibody, their adherence to OBA9 cells was not inhibited. Stimulation of OBA9 cells with purified Omp29 increased the phosphorylation of focal adhesion kinase (FAK), a pivotal cell-signaling molecule that can up-regulate actin rearrangement. Furthermore, Omp29 increased the formation of F-actin in OBA9 cells. The internalization of Omp29-coated beads and the entry of Aa into OBA9 were partially inhibited by treatment with PI3-kinase inhibitor (Wortmannin) and Rho GTPases inhibitor (EDIN), both known to convey FAK-signaling to actin-rearrangement. These results suggest that Omp29 is associated with the entry of Aa into gingival epithelial cells by up-regulating F-actin rearrangement via the FAK signaling pathway.
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Registered trials
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